Coomassie Staining as Loading Control in Western Blot Analysis

被引:383
作者
Welinder, Charlotte [1 ]
Ekblad, Lars [1 ]
机构
[1] Lund Univ, Dept Oncol, SE-22185 Lund, Sweden
关键词
Western blot; loading control; Coomassie; MALDI-TOF; PVDF; GAPDH; peptide mass fingerprinting; PROTEIN IDENTIFICATION; MEMBRANES; ACTIN;
D O I
10.1021/pr1011476
中图分类号
Q5 [生物化学];
学科分类号
070307 [化学生物学];
摘要
In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.
引用
收藏
页码:1416 / 1419
页数:4
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