Cloning and tissue distribution of a novel P2X receptor from rat brain

被引：82

作者：

Soto, M

GarciaGuzman, M

Karschin, C

Stuhmer, W

机构：

[1] Dept. of Molec. Biol. of Neuronal S., Max-Planck Inst. for Exp. Medicine, D-37075 Göttingen

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 223卷 / 02期

关键词：

D O I：

10.1006/bbrc.1996.0915

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated the cDNA for a novel member (P2X(6)) of the ATP-gated ion channel family. The rat P2X(6) nucleotide sequence encodes a 379 amino acid protein that conserves all the structural features of previously cloned P2X receptors, including the two putative transmembrane domains predicted by hydrophobicity plots. In situ hybridization analysis of rat brain sections showed a wide pattern of mRNA expression that is virtually identical to that already described for P2X(4). Injection of P2X(6) cRNA in Xenopus oocytes did not give rise to ATP-activated channels. Coexpression of P2X(6) with P2X(4) subunits produced currents which were not discernibly different from those of P2X(4) expressed alone. (C) 1996 Academic Press, Inc.

引用

页码：456 / 460

页数：5

共 17 条

[1]

BARTSCH S, 1992, J NEUROSCI, V12, P736

[2] A P2X PURINOCEPTOR CDNA CONFERRING A NOVEL PHARMACOLOGICAL PROFILE [J].