Electron transport routes in whole cells of Synechocystis sp strain PCC 6803:: The role of the cytochrome bd-type oxidase

被引:88
作者
Berry, S
Schneider, D
Vermaas, WFJ
Rögner, M
机构
[1] Ruhr Univ Bochum, Fak Biol, Lehrstuhl Biochem Pflanzen, D-44780 Bochum, Germany
[2] Arizona State Univ, Dept Plant Biol, Tempe, AZ 85287 USA
[3] Arizona State Univ, Ctr Study Early Events Photosynthesis, Tempe, AZ 85287 USA
关键词
D O I
10.1021/bi011683d
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The plastoquinone pool is the central switching point of both respiratory and photosynthetic electron transport in cyanobacteria. Its redox state can be monitored noninvasively in whole cells using chlorophyll fluorescence induction, avoiding possible artifacts associated with thylakoid membrane preparations. This method was applied to cells of Synechocystis sp. PCC 6803 to study respiratory reactions involving the plastoquinone pool. The role of the respiratory oxidases known from the genomic sequence of Synechocystis sp. PCC 6803 was investigated by a combined strategy using inhibitors and deletion strains that lack one or more of these oxidases. The putative quinol oxidase of the cytochrome bd-type was shown to participate in electron transport in thylakoid membranes. The activity of this enzyme in thylakoids was strongly dependent on culture conditions; it was increased under conditions where the activity of the cytochrome b(6)f complex alone may be insufficient for preventing over-reduction of the PQ pool. In contrast, no indication of quinol oxidase activity in thylakoids was found for a second alternative oxidase encoded by the ctall genes.
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页码:3422 / 3429
页数:8
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