Hippocampal LTD expression involves a pool of AMPARs regulated by the NSF-GluR2 interaction

被引：242

作者：

Lüthi, A ^{[1
]}

Chittajallu, R ^{[1
]}

Duprat, F ^{[1
]}

Palmer, MJ ^{[1
]}

Benke, TA ^{[1
]}

Kidd, FL ^{[1
]}

Henley, JM ^{[1
]}

Isaac, JTR ^{[1
]}

Collingridge, GL ^{[1
]}

机构：

[1] Univ Bristol, MRC, Dept Anat, Ctr Synapt Plast, Bristol BS8 1TD, Avon, England

来源：

NEURON | 1999年 / 24卷 / 02期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1016/S0896-6273(00)80852-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We investigated whether the interaction between the N-ethyl-maleimide-sensitive fusion protein (NSF) and the AMPA receptor (AMPAR) subunit GluR2 is involved in synaptic plasticity in the CA1 region of the hippocampus. Blockade of the NSF-GluR2 interaction by a specific peptide (pep2m) introduced into neurons prevented homosynaptic, de novo long-term depression (LTD). Moreover, saturation of LTD prevented the pep2m-induced reduction in AMPAR-mediated excitatory postsynaptic currents (EPSCs). Minimal stimulation experiments indicated that both pep2m action and LTD were due to changes in quantal size and quantal content but were not associated with changes in AMPAR single-channel conductance or EPSC kinetics. These results suggest that there is a pool of AMPARs dependent on the NSF-GluR2 interaction and that LTD expression involves the removal of these receptors from synapses.

引用

页码：389 / 399

页数：11

共 34 条

[1] Long-term depression in hippocampus [J].

Bear, MF ;

Abraham, WC .

ANNUAL REVIEW OF NEUROSCIENCE, 1996, 19 :437-462

[2] Modulation of AMPA receptor unitary conductance by synaptic activity [J].

Benke, TA ;

Lüthi, A ;

Isaac, JTR ;

Collingridge, GL .

NATURE, 1998, 393 (6687) :793-797

[3] NEUROTRANSMITTER RECEPTORS .2. AMPA AND KAINATE RECEPTORS [J].

BETTLER, B ;

MULLE, C .

NEUROPHARMACOLOGY, 1995, 34 (02) :123-139

[4] A SYNAPTIC MODEL OF MEMORY - LONG-TERM POTENTIATION IN THE HIPPOCAMPUS [J].

BLISS, TVP ;

COLLINGRIDGE, GL .

NATURE, 1993, 361 (6407) :31-39

[5] POSTSYNAPTIC INDUCTION AND PRESYNAPTIC EXPRESSION OF HIPPOCAMPAL LONG-TERM DEPRESSION [J].

BOLSHAKOV, VY ;

SIEGELBAUM, SA .

SCIENCE, 1994, 264 (5162) :1148-1152

[6]

BRAITHWAITE SP, 1999, IN PRESS NEUROPHARMA

[7] DIVALENT ION PERMEABILITY OF AMPA RECEPTOR CHANNELS IS DOMINATED BY THE EDITED FORM OF A SINGLE SUBUNIT [J].

BURNASHEV, N ;

MONYER, H ;

SEEBURG, PH ;

SAKMANN, B .

NEURON, 1992, 8 (01) :189-198

[8] Rapid redistribution of glutamate receptors contributes to long-term depression in hippocampal cultures [J].

Carroll, RC ;

Lissin, DV ;

von Zastrow, M ;

Nicoll, RA ;

Malenka, RC .

NATURE NEUROSCIENCE, 1999, 2 (05) :454-460

[9] PDZ proteins organize synaptic signaling pathways [J].

Craven, SE ;

Bredt, DS .

CELL, 1998, 93 (04) :495-498

[10] The protein kinase Cα binding protein PICK1 interacts with short but not long form alternative splice variants of AMPA receptor subunits [J].

Dev, KK ;

Nishimune, A ;

Henley, JM ;

Nakanishi, S .

NEUROPHARMACOLOGY, 1999, 38 (05) :635-644

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