Hippocampal LTD expression involves a pool of AMPARs regulated by the NSF-GluR2 interaction

被引：242

作者：

Lüthi, A ^{[1
]}

Chittajallu, R ^{[1
]}

Duprat, F ^{[1
]}

Palmer, MJ ^{[1
]}

Benke, TA ^{[1
]}

Kidd, FL ^{[1
]}

Henley, JM ^{[1
]}

Isaac, JTR ^{[1
]}

Collingridge, GL ^{[1
]}

机构：

[1] Univ Bristol, MRC, Dept Anat, Ctr Synapt Plast, Bristol BS8 1TD, Avon, England

来源：

NEURON | 1999年 / 24卷 / 02期

基金：

英国医学研究理事会; 英国惠康基金;

关键词：

D O I：

10.1016/S0896-6273(00)80852-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We investigated whether the interaction between the N-ethyl-maleimide-sensitive fusion protein (NSF) and the AMPA receptor (AMPAR) subunit GluR2 is involved in synaptic plasticity in the CA1 region of the hippocampus. Blockade of the NSF-GluR2 interaction by a specific peptide (pep2m) introduced into neurons prevented homosynaptic, de novo long-term depression (LTD). Moreover, saturation of LTD prevented the pep2m-induced reduction in AMPAR-mediated excitatory postsynaptic currents (EPSCs). Minimal stimulation experiments indicated that both pep2m action and LTD were due to changes in quantal size and quantal content but were not associated with changes in AMPAR single-channel conductance or EPSC kinetics. These results suggest that there is a pool of AMPARs dependent on the NSF-GluR2 interaction and that LTD expression involves the removal of these receptors from synapses.

引用

页码：389 / 399

页数：11

共 34 条

[11] GRIP: A synaptic PDZ domain-containing protein that interacts with AMPA receptors [J].

Dong, HL ;

OBrien, RJ ;

Fung, ET ;

Lanahan, AA ;

Worley, PF ;

Huganir, RL .

NATURE, 1997, 386 (6622) :279-284

[12] HOMOSYNAPTIC LONG-TERM DEPRESSION IN AREA CA1 OF HIPPOCAMPUS AND EFFECTS OF N-METHYL-D-ASPARTATE RECEPTOR BLOCKADE [J].

DUDEK, SM ;

BEAR, MF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (10) :4363-4367

[13] RELATIVE ABUNDANCE OF SUBUNIT MESSENGER-RNAS DETERMINES GATING AND CA2+ PERMEABILITY OF AMPA RECEPTORS IN PRINCIPAL NEURONS AND INTERNEURONS IN RAT CNS [J].

GEIGER, JRP ;

MELCHER, T ;

KOH, DS ;

SAKMANN, B ;

SEEBURG, PH ;

JONAS, P ;

MONYER, H .

NEURON, 1995, 15 (01) :193-204

[14] Synaptic refractory period provides a measure of probability of release in the hippocampus [J].

Hjelmstad, GO ;

Nicoll, RA ;

Malenka, RC .

NEURON, 1997, 19 (06) :1309-1318

[15] CLONED GLUTAMATE RECEPTORS [J].

HOLLMANN, M ;

HEINEMANN, S .

ANNUAL REVIEW OF NEUROSCIENCE, 1994, 17 :31-108

[16] Long-term potentiation at single fiber inputs to hippocampal CA1 pyramidal cells [J].

Isaac, JTR ;

Hjelmstad, GO ;

Nicoll, RA ;

Malenka, RC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (16) :8710-8715

[17] An investigation of the expression mechanism of LTP of AMPA receptor-mediated synaptic transmission at hippocampal CA1 synapses using failures analysis and dendritic recordings [J].

Isaac, JTR ;

Lüthi, A ;

Palmer, MJ ;

Anderson, WW ;

Benke, TA ;

Collingridge, GL .

NEUROPHARMACOLOGY, 1998, 37 (10-11) :1399-1410

[18] Enhanced LTP in mice deficient in the AMPA receptor GluR2 [J].

Jia, ZP ;

Agopyan, N ;

Miu, P ;

Xiong, ZG ;

Henderson, J ;

Gerlai, R ;

Taverna, FA ;

Velumian, A ;

MacDonald, J ;

Carlen, P ;

AbramowNewerly, W ;

Roder, J .

NEURON, 1996, 17 (05) :945-956

[19] DIFFERENCES IN CA2+ PERMEABILITY OF AMPA-TYPE GLUTAMATE-RECEPTOR CHANNELS IN NEOCORTICAL NEURONS CAUSED BY DIFFERENTIAL GLUR-B SUBUNIT EXPRESSION [J].

JONAS, P ;

RACCA, C ;

SAKMANN, B ;

SEEBURG, PH ;

MONYER, H .

NEURON, 1994, 12 (06) :1281-1289

[20] MECHANISMS UNDERLYING INDUCTION OF HOMOSYNAPTIC LONG-TERM DEPRESSION IN AREA CA1 OF THE HIPPOCAMPUS [J].

MULKEY, RM ;

MALENKA, RC .

NEURON, 1992, 9 (05) :967-975

← 1 2 3 4 →