Systemically Injected Exosomes Targeted to EGFR Deliver Antitumor MicroRNA to Breast Cancer Cells

被引：1468

作者：

Ohno, Shin-ichiro

Takanashi, Masakatsu

Sudo, Katsuko ^{[2
]}

Ueda, Shinobu

Ishikawa, Akio

Matsuyama, Nagahisa

Fujita, Koji

Mizutani, Takayuki

Ohgi, Tadaaki ^{[3
]}

Ochiya, Takahiro ^{[4
]}

Gotoh, Noriko ^{[5
]}

Kuroda, Masahiko ^{[1
]}

机构：

[1] Tokyo Med Univ, Dept Mol Pathol, Shinjuku Ku, Tokyo 1608402, Japan

[2] Tokyo Med Univ, Anim Res Ctr, Tokyo 1608402, Japan

[3] BONAC Corp, Fukuoka BIO Factory 4F, Fukuoka, Japan

[4] Natl Canc Ctr, Res Inst, Div Mol & Cellular Med, Tokyo 104, Japan

[5] Univ Tokyo, Inst Med Sci, Div Syst Biomed Technol, Tokyo, Japan

来源：

MOLECULAR THERAPY | 2013年 / 21卷 / 01期

关键词：

GROWTH-FACTOR RECEPTOR; PEPTIDE LIGAND; LET-7; GENE; EXPRESSION; REPRESSES;

D O I：

10.1038/mt.2012.180

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 [微生物学]; 090105 [作物生产系统与生态工程];

摘要：

Despite the therapeutic potential of nucleic acid drugs, their clinical application has been limited in part by a lack of appropriate delivery systems. Exosomes or microvesicles are small endosomally derived vesicles that are secreted by a variety of cell types and tissues. Here, we show that exosomes can efficiently deliver microRNA (miRNA) to epidermal growth factor receptor (EGFR)-expressing breast cancer cells. Targeting was achieved by engineering the donor cells to express the transmembrane domain of platelet-derived growth factor receptor fused to the GE11 peptide. Intravenously injected exosomes delivered let-7a miRNA to EGFR-expressing xenograft breast cancer tissue in RAG2(-/-) mice. Our results suggest that exosomes can be used therapeutically to target EGFR-expressing cancerous tissues with nucleic acid drugs.

引用

页码：185 / 191

页数：7

共 21 条

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Microvesicle-mediated RNA Molecule Delivery System Using Monocytes/Macrophages [J].