microRNA-9 targets the long non-coding RNA MALAT1 for degradation in the nucleus

被引：212

作者：

Leucci, Eleonora ^{[1
,2
]}

Patella, Francesca ^{[1
,2
,3
]}

Waage, Johannes ^{[1
,2
,4
]}

Holmstrom, Kim ^{[6
]}

Lindow, Morten ^{[7
]}

Porse, Bo ^{[1
,2
,4
,5
]}

Kauppinen, Sakari ^{[8
]}

Lund, Anders H. ^{[1
,2
]}

机构：

[1] Univ Copenhagen, Biotech Res & Innovat Ctr, DK-1168 Copenhagen, Denmark

[2] Univ Copenhagen, Ctr Epigenet, DK-1168 Copenhagen, Denmark

[3] Scuola Super Sant Anna, Pisa, Italy

[4] Univ Copenhagen, Fac Hlth Sci, Rigshosp, Finsen Lab, DK-1168 Copenhagen, Denmark

[5] Univ Copenhagen, Fac Hlth Sci, Danish Stem Cell Ctr DanStem, DK-1168 Copenhagen, Denmark

[6] Bioneer AS, Horsholm, Denmark

[7] Santaris Pharma, Horsholm, Denmark

[8] Aalborg Univ Hosp, Dept Haematol, Aalborg, Denmark

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

关键词：

IN-VIVO; EVOLUTION; MIR-9;

D O I：

10.1038/srep02535

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

microRNAs regulate the expression of over 60% of protein coding genes by targeting their mRNAs to AGO2-containing complexes in the cytoplasm and promoting their translational inhibition and/or degradation. There is little evidence so far for microRNA-mediated regulation of other classes of non-coding RNAs. Here we report that microRNA-9 (miR-9) regulates the expression of the Metastasis Associated Lung Adenocarcinoma Transcript 1 (MALAT-1), one of the most abundant and conserved long non-coding RNAs. Intriguingly, we find that miR-9 targets AGO2-mediated regulation of MALAT1 in the nucleus. Our findings reveal a novel direct regulatory link between two important classes of non-coding RNAs, miRs and lncRNAs, and advance our understanding of microRNA functions.

引用

页数：6

共 36 条

[21] A large noncoding RNA is a marker for murine hepatocellular carcinomas and a spectrum of human carcinomas
Lin, R.
Maeda, S.
Liu, C.
Karin, M.
Edgington, T. S.
[J]. ONCOGENE, 2007, 26 (06) : 851 - 858
[22] Control of RNA processing by a large non-coding RNA over-expressed in carcinomas
Lin, Rui
Roychowdhury-Saha, Manami
Black, Chris
Watt, Andrew T.
Marcusson, Eric G.
Freier, Susan M.
Edgington, Thomas S.
[J]. FEBS LETTERS, 2011, 585 (04): : 671 - 676
[23] Malat1 is not an essential component of nuclear speckles in mice
Nakagawa, Shinichi
Ip, Joanna Y.
Shioi, Go
Tripathi, Vidisha
Zong, Xinying
Hirose, Tetsuro
Prasanth, Kannanganattu V.
[J]. RNA, 2012, 18 (08) : 1487 - 1499
[24] Fluorescence correlation spectroscopy and fluorescence cross-correlation spectroscopy reveal the cytoplasmic origination of loaded nuclear RISC in vivo in human cells
Ohrt, Thomas
Muetze, Joerg
Staroske, Wolfgang
Weinmann, Lasse
Hoeck, Julia
Crell, Karin
Meister, Gunter
Schwille, Petra
[J]. NUCLEIC ACIDS RESEARCH, 2008, 36 (20) : 6439 - 6449
[25] Immunoprecipitation of mRNA-protein complexes
Peritz, Tiina
Zeng, Fanyi
Kannanayakal, Theresa J.
Kilk, Kalle
Eiriksdottir, Emelia
Langel, Ulo
Eberwine, James
[J]. NATURE PROTOCOLS, 2006, 1 (02) : 577 - 580
[26] Fast and effective prediction of microRNA/target duplexes
Rehmsmeier, M
Steffen, P
Höchsmann, M
Giegerich, R
[J]. RNA, 2004, 10 (10) : 1507 - 1517
[27] A ceRNA Hypothesis: The Rosetta Stone of a Hidden RNA Language?
Salmena, Leonardo
Poliseno, Laura
Tay, Yvonne
Kats, Lev
Pandolfi, Pier Paolo
[J]. CELL, 2011, 146 (03) : 353 - 358
[28] A Sensitive Alternative for MicroRNA In Situ Hybridizations Using Probes of 2′-O-Methyl RNA plus LNA
Soe, Martin J.
Moller, Trine
Dufva, Martin
Holmstrom, Kim
[J]. JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 2011, 59 (07) : 661 - 672
[29] Transcript assembly and quantification by RNA-Seq reveals unannotated transcripts and isoform switching during cell differentiation
Trapnell, Cole
Williams, Brian A.
Pertea, Geo
Mortazavi, Ali
Kwan, Gordon
van Baren, Marijke J.
Salzberg, Steven L.
Wold, Barbara J.
Pachter, Lior
[J]. NATURE BIOTECHNOLOGY, 2010, 28 (05) : 511 - U174
[30] The Nuclear-Retained Noncoding RNA MALAT1 Regulates Alternative Splicing by Modulating SR Splicing Factor Phosphorylation
Tripathi, Vidisha
Ellis, Jonathan D.
Shen, Zhen
Song, David Y.
Pan, Qun
Watt, Andrew T.
Freier, Susan M.
Bennett, C. Frank
Sharma, Alok
Bubulya, Paula A.
Blencowe, Benjamin J.
Prasanth, Supriya G.
Prasanth, Kannanganattu V.
[J]. MOLECULAR CELL, 2010, 39 (06) : 925 - 938

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