Mammalian transgenesis by intracytoplasmic sperm injection

被引：330

作者：

Perry, ACF ^{[1
]}

Wakayama, T

Kishikawa, H

Kasai, T

Okabe, M

Toyoda, Y

Yanagimachi, R

机构：

[1] Univ Hawaii, John A Burns Sch Med, Dept Anat & Reprod Biol, Honolulu, HI 96822 USA

[2] Osaka Univ, Genome Informat Res Ctr, Osaka 5650871, Japan

[3] Obihiro Univ Agr & Vet Med, Res Ctr Protozoan Mol Immunol, Obihiro, Hokkaido 080, Japan

来源：

SCIENCE | 1999年 / 284卷 / 5417期

关键词：

D O I：

10.1126/science.284.5417.1180

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Coinjection of-unfertilized mouse oocytes with sperm heads and exogenous DNA encoding either a green fluorescent protein (GFP) or beta-galactosidase reporter produced 64 to 94 percent transgene-expressing embryos, reflecting DNA-sperm head association before coinjection. Nonselective transfer to surrogate mothers of embryos in the GFP series generated about 20 percent offspring expressing the integrated transgene. These data indicate that exogenous DNA can reproducibly be delivered into an oocyte by microinjected spermatozoa and suggest an adaptable method of transgenesis.

引用

页码：1180 / 1183

页数：4

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