The effect of high-fat feeding on intramuscular lipid and lipid peroxidation levels in UCP3-ablated mice

被引：22

作者：

Hoeks, J

Hesselink, MKC

Sluiter, W

Schaart, G

Willems, J

Morrisson, A

Clapham, JC

Saris, WHM

Schrauwen, P

机构：

[1] Maastricht Univ, Dept Human Biol, Nutr & Toxicol Res Inst Maastricht, NL-6200 MD Maastricht, Netherlands

[2] Maastricht Univ, Dept Movement Sci, Nutr & Toxicol Res Inst Maastricht, NL-6200 MD Maastricht, Netherlands

[3] Erasmus MC, Dept Biochem, Rotterdam, Netherlands

[4] GlaxoSmithKline, Dept Comparat Genom, Harlow, Essex, England

[5] GlaxoSmithKline, Dept Vasc Biol, Harlow, Essex, England

来源：

FEBS LETTERS | 2006年 / 580卷 / 05期

关键词：

mitochondrion; lipotoxicity; reactive oxygen species; UCP3; high fat diet;

D O I：

10.1016/j.febslet.2006.01.059

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Uncoupling protein-3 (UCP3) has been suggested to protect against lipid-induced oxidative damage. Therefore, we studied intramuscular lipid peroxide levels and high-fat diet induced alterations in muscle lipid metabolism of UCP3-ablated mice. UCP3(-/-) mice showed similar to 3-fold higher levels of intramuscular lipid peroxides upon standard chow feeding, compared to wildtype littermates. Remarkably, this difference was no longer apparent on the high-fat diet. However, upon high-fat feeding, intramuscular triacylglycerol levels were similar to 50% lower in UCP3(-/-) mice, in comparison to UCP3(+/+) animals. Succinate dehydrogenase activity, and total protein content of the muscle fatty acid transporter FAT/CD36 were however similar between UCP3(-/-) and UCP3(+/+) mice. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1371 / 1375

页数：5

共 33 条

[1] Effects of fasting on muscle mitochondrial energetics and fatty acid metabolism in Ucp3(-/-) and wild-type mice [J].