Detection, localization, and conformational analysis of single polysaccharide molecules on live bacteria

被引：138

作者：

Francius, Gregory ^{[1
]}

Lebeer, Sarah ^{[2
,3
]}

Alsteens, David ^{[1
]}

Wildling, Linda ^{[4
]}

Gruber, Hermann J. ^{[4
]}

Hols, Pascal ^{[5
]}

De Keersmaecker, Sigrid ^{[2
,3
]}

Vanderleyden, Jos ^{[2
,3
]}

Dufrene, Yves F. ^{[1
]}

机构：

[1] Catholic Univ Louvain, Unite Chim Interfaces, B-1348 Louvain, Belgium

[2] Katholieke Univ Leuven, Ctr Microbial & Plant Genet, B-3001 Louvain, Belgium

[3] Katholieke Univ Leuven, INPAC, B-3001 Louvain, Belgium

[4] Johannes Kepler Univ Linz, Inst Biophys, A-4040 Linz, Austria

[5] Catholic Univ Louvain, Unite Genet, Inst Sci Vie, B-1348 Louvain, Belgium

来源：

ACS NANO | 2008年 / 2卷 / 09期

关键词：

atomic force microscopy; elasticity; bacteria; lectins; mutants; polysaccharides; single-molecule force spectroscopy;

D O I：

10.1021/nn800341b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The nanoscale exploration of microbes using atomic force microscopy (AFM) is an exciting, rapidly evolving research field. Here, we show that single-molecule force spectroscopy is a valuable tool for the localization and conformational analysis of individual polysaccharides on live bacteria. We focus on the clinically important probiotic bacterium Lactobacillus rhamnosus GG, demonstrating the power of AFM to reveal the coexistence of polysaccharide chains of different nature on the cell surface. Applicable to a wide variety of cells, this single molecule method offers exciting prospects for analyzing the heterogeneity and diversity of macromolecules constituting cell membranes and cell walls.

引用

页码：1921 / 1929

页数：9

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