Development of amperometric glucose biosensor based on glucose oxidase co-immobilized with multi-walled carbon nanotubes at low potential

被引：111

作者：

Rahman, M. M. ^{[1
]}

Umar, Ahmad ^{[2
]}

Sawada, Kazuaki ^{[1
]}

机构：

[1] Toyohashi Univ Technol, Dept Elect & Elect Engn, Venture Business Lab, Aichi 4418580, Japan

[2] Chonbuk Natl Univ, Dept Chem Engn, Jeonju 561756, South Korea

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2009年 / 137卷 / 01期

关键词：

MWCNT; Glucose oxidase; Cyclic voltammetry; Chronoamperometry; ELECTRON-TRANSFER KINETICS; TRANSFER RATE CONSTANTS; SOL-GEL; ELECTROCHEMICAL-BEHAVIOR; ADSORBED MOLECULES; ORDERED GRAPHITE; REDOX PROTEINS; PART; ENZYMES; SENSORS;

D O I：

10.1016/j.snb.2008.10.060

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The electrodes modified with multi-walled carbon nanotube (MWCNT) film containing adsorbed glucose oxidase (GOx) with respect to highly sensitive glucose detection are developed and demonstrated by electrochemical studies at low potential. Glucose-sensing properties were studied using cyclic voltammetric and chronoamperometric techniques. A linear calibration plot was obtained in the concentration range between 1.0 and 500.0 mu M, and the detection limit was determined to be 1.3 +/- 0.1 mu M. Interferences from other biological compounds were studied. The long-term stability, reproducibility, and sensitivity of the GOx biosensor were measured. The proposed glucose biosensor was successfully applied to human serum sample. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：327 / 333

页数：7

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