Two-photon fluorescence surface wave microscopy

被引:7
作者
Goh, JYL
Somekh, MG
See, CW
Pitter, MC
Vere, KA
O'Shea, P
机构
[1] Sch Elect & Elect Engn, Appl Opt Grp, Nottingham NG7 2RD, England
[2] Queens Med Ctr, Sch Biol, Cell Biophys Grp, Sch Med, Nottingham NG7 2RD, England
基金
英国惠康基金;
关键词
cell membrane; evanescent wave; microscopy; surface plasmon; surface wave; two-photon;
D O I
10.1111/j.1365-2818.2005.01525.x
中图分类号
TH742 [显微镜];
学科分类号
摘要
This paper demonstrates the principle of two-photon surface wave microscopy with a view to applications on biological samples. We describe a modified scanning optical microscope, which uses specially prepared coverslips. These coverslips are designed to support the propagation of surface waves capable of large field enhancements. We also discuss the beam conditioning necessary to ensure efficient use of the available illumination. Two-photon surface wave fluorescent excitation is demonstrated on fluorescent nanospheres, demonstrating a point spread function width of approximate to 220 nm at an illumination wavelength of 925 nm. The potential of non-linear surface wave excitation for both fluorescence and harmonic imaging microscopy is discussed.
引用
收藏
页码:168 / 175
页数:8
相关论文
共 15 条
[1]   Total internal reflection fluorescence microscopy in cell biology [J].
Axelrod, D .
TRAFFIC, 2001, 2 (11) :764-774
[2]  
AZZAM RMA, 1986, ELLIPSOMETRY POLARIZ, P332
[3]   DIFFRACTION-FREE BEAMS [J].
DURNIN, J ;
MICELI, JJ ;
EBERLY, JH .
PHYSICAL REVIEW LETTERS, 1987, 58 (15) :1499-1501
[4]   LATERAL DISPLACEMENT AND DISTORTION OF BEAMS INCIDENT UPON A TRANSMITTING-LAYER CONFIGURATION [J].
HSUE, CW ;
TAMIR, T .
JOURNAL OF THE OPTICAL SOCIETY OF AMERICA A-OPTICS IMAGE SCIENCE AND VISION, 1985, 2 (06) :978-988
[5]   OPTICAL CONSTANTS OF NOBLE METALS [J].
JOHNSON, PB ;
CHRISTY, RW .
PHYSICAL REVIEW B, 1972, 6 (12) :4370-4379
[6]   Stimulated emission depletion microscopy with an offset depleting beam [J].
Klar, TA ;
Dyba, M ;
Hell, SW .
APPLIED PHYSICS LETTERS, 2001, 78 (04) :393-395
[7]   Multiparameter evanescent-wave Imaging in biological fluorescence microscopy [J].
Oheim, M ;
Stühmer, W .
IEEE JOURNAL OF QUANTUM ELECTRONICS, 2002, 38 (02) :142-148
[8]   Nonlinear optical measurement of membrane potential around single molecules at selected cellular sites [J].
Peleg, G ;
Lewis, A ;
Linial, M ;
Loew, LM .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (12) :6700-6704
[9]   Focusing light to a tighter spot [J].
Quabis, S ;
Dorn, R ;
Eberler, M ;
Glöckl, O ;
Leuchs, G .
OPTICS COMMUNICATIONS, 2000, 179 (1-6) :1-7
[10]  
SALEH BEA, 1991, FUNDAMENTALS PHOTONI, P255