Miniaturized biosensor for avian influenza virus detection

被引：59

作者：

Diouani, M. F. ^{[2
]}

Helali, S. ^{[1
]}

Hafaid, I. ^{[1
,4
]}

Hassen, W. M. ^{[1
,4
]}

Snoussi, M. A. ^{[2
]}

Ghram, A. ^{[3
]}

Jaffrezic-Renault, N. ^{[4
]}

Abdelghani, A. ^{[1
]}

机构：

[1] IPEST, Unite Rech Phys Semicond & Capteurs, Tunis 2070, Tunisia

[2] Inst Pasteur Tunis, Grp Vet Serv Epidemiol Med, Tunis, Tunisia

[3] Inst Pasteur Tunis, Lab Microbiol Vet, Tunis, Tunisia

[4] Univ Lyon 1, CNRS, Sci Analyt Lab, UMR 5180, F-69622 Villeurbanne, France

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2008年 / 28卷 / 5-6期

关键词：

pathogenic avian influenza; biosensors; impedance spectroscopy;

D O I：

10.1016/j.msec.2007.10.043

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Avian Influenza Virus (AIV) infections are a major cause of mortality and rapid identification of the virus has important clinical, economical and epidemiological implications The traditional methods for avian influenza vir-us diagnostic are Enzyme Linked Immunosorbent Assay (ELISA) and Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) which are time consuming and expensive. In this paper we describe the development of miniaturized gold electrode biosensor for H7N1 detection with electrochemical technique such us impedance spectroscopy. The biosensor is based on the immobilization of specific H7N1 antibodies onto biofunctionnalized gold electrode. Each grafted layer on the gold electrode was characterized with impedance spectroscopy and modelised with an equivalent electric circuit. The affinity interaction of the antibody with the specific antigen can be measured with a low limit detection (5 mu g/ml) and with a good reproductibility. The non-specific interaction has been tested with the Newcastle antigen. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：580 / 583

页数：4

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