Electrical and optical characterization of thrombin-induced permeability of cultured endothelial cell monolayers on semiconductor electrode arrays

被引：34

作者：

Hillebrandt, H

Abdelghani, A

Abdelghani-Jacquin, C

Aepfelbacher, M

Sackmann, E

机构：

[1] Tech Univ Munich, Dept Phys E22, Inst Biophys, D-85748 Garching, Germany

[2] IPEST, Uniti Rech Phys Semicond, La Marsa 2070, Tunisia

[3] Univ Munich, Max von Pettenkofer Inst Med Mikrobiol, D-80336 Munich, Germany

来源：

APPLIED PHYSICS A-MATERIALS SCIENCE & PROCESSING | 2001年 / 73卷 / 05期

关键词：

D O I：

10.1007/s003390100879

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Impedance spectroscopy and phase-contrast microscopy are combined to monitor the electrical and morphological properties of human umbilical vein endothelial cell monolayers. The cells were cultured on optically transparent indium-tin-oxide (ITO) semiconductor electrode arrays coated with collagen IV, and the effect of the inflammatory mediator thrombin on monolayer permeability was monitored in real time. ITO electrodes provide several advantages for these kinds of experiments, because they are optically transparent, polarizable and highly sensitive due to the absence of insulating oxide layers. A qualitative correlation between the thrombin-induced gap formation and the electrical parameters of the cell layer is established.

引用

页码：539 / 546

页数：8

共 39 条

[1] CULTURE OF MAMMALIAN-CELLS ON POLYPYRROLE-COATED ITO AS A BIOCOMPATIBLE ELECTRODE [J].