Monitoring of cellular behaviour by impedance measurements on interdigitated electrode structures

被引：323

作者：

Ehret, R ^{[1
]}

Baumann, W ^{[1
]}

Brischwein, M ^{[1
]}

Schwinde, A ^{[1
]}

Stegbauer, K ^{[1
]}

Wolf, B ^{[1
]}

机构：

[1] UNIV FREIBURG,FAK MED,INST IMMUNOBIOL,AG MED PHYS & ELEKT MIKROSKOPIE,D-79104 FREIBURG,GERMANY

来源：

BIOSENSORS & BIOELECTRONICS | 1997年 / 12卷 / 01期

关键词：

cellular biosensor; interdigitated electrode structures; impedance; cytotoxicity testing; serum components; PhysicoControl-Microsystem;

D O I：

10.1016/0956-5663(96)89087-7

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A new method for on-line and real-time monitoring of concentration, growth and physiological state of cells in culture is described. This biosensor is based on impedance measurements of adherently growing cells on interdigitated electrode structures (IDES). The measurements can be performed for several days as there is no detectable electrical influence on the cells. The versatility of this new sensor is shown with some exemplary experiments. Cell density, growth and long-term behaviour of cells on the electrodes clearly change the impedance of the IDES. Both, the global influence of serum components (deprivation of foetal bovine serum) and the toxic effects of heavy metal ions (cadmium) result in changes of the sensor signal and can be visualized. (C) 1996 Elsevier Science Limited

引用

页码：29 / 41

页数：13

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