Impedance spectroscopy and affinity measurement of specific antibody-antigen interaction

被引：42

作者：

Tlili, A

Abdelghani, A

Ameur, S

Jaffrezic-Renault, N

机构：

[1] IPEST, Unite Rech Phys Semicond & Capteurs, Tunis 2070, Tunisia

[2] ECL, CEGELY, Ctr Genie Elect Lyon, F-69134 Ecully, France

来源：

MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS | 2006年 / 26卷 / 2-3期

关键词：

biosensors; impedance spectroscopy; QCM; affinity;

D O I：

10.1016/j.msec.2005.10.007

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

We use impedance spectroscopy technique to develop a rapid and sensitive antigen biosensor. The biosensor is based on the immobilization of antibodies onto an 11-Mercaptoundecanoic acid self-assembled monolayer. The high insulating properties of the thiol can be measured with cyclic voltammetry and impedance spectroscopy with a redox couple. The, the modified gold electrode is activated with 2-ethyl-3 (3-dimethyl aminopropyl) carbodimude (EDC) and N-hydroxy succimmide (NES) ester for antibody immobilisation. The affinity interaction of the antigen-antibody can be measured with quartz crystal microbalance technique. Dissociation and association constant are determined by applying a Fortran program fitting for the experimental data. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：546 / 550

页数：5

共 24 条

[1] Evaluation of antibody immobilization methods for piezoelectric biosensor application [J].