Quartz crystal microbalance (QCM) as a device for the screening of phage libraries

被引：38

作者：

Hengerer, A

Decker, J

Prohaska, E

Hauck, S

Kösslinger, C

Wolf, H

机构：

[1] Univ Regensburg, Inst Med Microbiol & Hyg, D-93053 Regensburg, Germany

[2] Fraunhofer Inst Solid State Technol, D-80686 Munich, Germany

来源：

BIOSENSORS & BIOELECTRONICS | 1999年 / 14卷 / 02期

关键词：

immunosensors; quartz crystal microbalance; recombinant antibodies; phage display; single chain antibodies; human pancreatic secretory trypsin inhibitor;

D O I：

10.1016/S0956-5663(98)00111-0

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

An immunosensing system based on a quartz crystal microbalance (QCM) is presented for the selection of both antigen specific recombinant antibodies and antigen specific human pancreatic secretory trypsin inhibitor (hPSTI) mutants isolated from large phage libraries. The QCM was integrated into a flow injection analysis system for the straightforward analysis of large sample numbers. Measurements were performed using a biotinylated antigen immobilized by streptavidin onto the gold surface of the quartz crystal and phages displaying recombinant antibodies or hPSTI mutants. The results obtained by the QCM were in accordance to those of a well established enzyme linked immunosorbent assay (ELISA). Therefore, the QCM is well suited for the detection of single high affinity clones isolated from large phage display libraries. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：139 / 144

页数：6

共 25 条

[1] HIV SEROLOGY USING PIEZOELECTRIC IMMUNOSENSORS [J].