Bruch membrane aging alters the gene expression profile of human retinal pigment epithelium

被引：24

作者：

Cai, H ^{[1
]}

Del Priore, LV ^{[1
]}

机构：

[1] Columbia Univ, Harkness Eye Inst Columbia, Dept Ophthalmol, New York, NY 10025 USA

来源：

CURRENT EYE RESEARCH | 2006年 / 31卷 / 02期

关键词：

age-related macular degeneration; Bruch membrane aging; gene expression; RPE;

D O I：

10.1080/02713680500514628

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

We investigated the effects of age-related changes within the Bruch membrane on the gene expression profile of the RPE. Immortalized human ARPE-19 cells were seeded onto acellular human Bruch membrane from younger and older donors and harvested 72 hr later; total RNA was isolated and the gene expression profile was determined using the Affymetrix Human Genome U95A gene chip. Twelve genes were upregulated and 8 genes were downregulated with Bruch membrane aging; RT-PCR confirms that Bruch membrane aging upregulates genes in RPE cells encoding for transforming growth factor alpha and downregulates genes for vitronectin and the membrane transporter ABCC5. The role of these changes in the pathogenesis of age-related diseases such as macular degeneration remains to be elucidated.

引用

页码：181 / 189

页数：9

共 51 条

[1] Drusen in age-related macular degeneration: Pathogenesis, natural course, and laser photocoagulation-induced regression [J].

Abdelsalam, A ;

Del Priore, L ;

Zarbin, MA .

SURVEY OF OPHTHALMOLOGY, 1999, 44 (01) :1-29

[2] A photoreceptor cell-specific ATP-binding transporter gene (ABCR) is mutated in recessive Stargardt macular dystrophy (vol 15, pg 236, 1997) [J].

Allikmets, R .

NATURE GENETICS, 1997, 17 (01) :122-122

[3] Mutation of the Stargardt disease gene (ABCR) in age-related macular degeneration [J].

Allikmets, R ;

Shroyer, NF ;

Singh, N ;

Seddon, JM ;

Lewis, RA ;

Bernstein, PS ;

Peiffer, A ;

Zabriskie, NA ;

Li, YX ;

Hutchinson, A ;

Dean, M ;

Lupski, JR ;

Leppert, M .

SCIENCE, 1997, 277 (5333) :1805-1807

[4]

Anderson DH, 1999, INVEST OPHTH VIS SCI, V40, P3305

[5] Contribution of glial cells and pericytes to the mRNA profiles of P-glycoprotein and multidrug resistance-associated proteins in an in vitro model of the blood-brain barrier [J].

Berezowski, V ;

Landry, C ;

Dehouck, MP ;

Cecchelli, R ;

Fenart, L .

BRAIN RESEARCH, 2004, 1018 (01) :1-9

[6]

Bernstein PS, 2002, INVEST OPHTH VIS SCI, V43, P466

[7] An optimized protocol for first strand cDNA synthesis from laser capture microdissected tissue [J].

Boylan, S ;

Honda, S ;

Hjelmeland, LM ;

Handa, JT .

LABORATORY INVESTIGATION, 2001, 81 (08) :1167-1169

[8]

Buraczynska M, 2002, INVEST OPHTH VIS SCI, V43, P603

[9]

CALDWELL RB, 1989, CURR EYE RES, V8, P907

[10] Drusen proteome analysis: An approach to the etiology of age-related macular degeneration [J].

Crabb, JW ;

Miyagi, M ;

Gu, XR ;

Shadrach, K ;

West, KA ;

Sakaguchi, H ;

Kamei, M ;

Hasan, A ;

Yan, L ;

Rayborn, ME ;

Salomon, RG ;

Hollyfield, JG .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (23) :14682-14687

← 1 2 3 4 5 6 →