PNA technology

被引：177

作者：

Nielsen, PE ^{[1
]}

机构：

[1] Univ Copenhagen, Panum Inst, Ctr Biomol Recognit, IMBG, DK-2200 Copenhagen, Denmark

来源：

MOLECULAR BIOTECHNOLOGY | 2004年 / 26卷 / 03期

关键词：

peptide nucleic acid; gene targeting; antisense; genetic diagnostics; DNA recognition;

D O I：

10.1385/MB:26:3:233

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Peptide nucleic acids (PNA) are deoxyribonucleic acid (DNA) mimics with a pseudopeptide backbone. PNA is an extremely good structural mimic of DNA (or of ribonucleic acid [RNA]), and PNA oligomers are able to form very stable duplex structures with Watson-Crick complementary DNA and RNA (or PNA) oligomers, and they can also bind to targets in duplex DNA by helix invasion. Therefore, these molecules are of interest in many areas of chemistry, biology, and medicine, including drug discovery, genetic diagnostics, molecular recognition, and the origin of life. Recent progress in studies of PNA properties and applications is reviewed.

引用

页码：233 / 248

页数：16

共 136 条

[1] Down-regulation of amyloid precursor protein by peptide nucleic acid oligomer in cultured rat primary neurons and astrocytes
Adlerz, L
Soomets, U
Holmlund, L
Viirlaid, S
Langel, Ü
Iverfeldt, K
[J]. NEUROSCIENCE LETTERS, 2003, 336 (01) : 55 - 59
[2] A peptide nucleic acid (PNA) is more rapidly internalized in cultured neurons when coupled to a retro-inverso delivery peptide.: The antisense activity depresses the target mRNA and protein in magnocellular oxytocin neurons
Aldrian-Herrada, G
Desarménien, MG
Orcel, H
Boissin-Agasse, L
Méry, J
Brugidou, J
Rabié, A
[J]. NUCLEIC ACIDS RESEARCH, 1998, 26 (21) : 4910 - 4916
[3] Synthesis of amino- and guanidino-G-clamp PNA monomers
Ausín, C
Ortega, JA
Robles, J
Grandas, A
Pedroso, E
[J]. ORGANIC LETTERS, 2002, 4 (23) : 4073 - 4075
[4] Sensitive detection of p53 gene mutations by a 'mutant enriched' PCR-SSCP technique
Behn, M
Schuermann, M
[J]. NUCLEIC ACIDS RESEARCH, 1998, 26 (05) : 1356 - 1358
[5] Behn M, 2000, J PATHOL, V190, P69
[6] Peptide nucleic acid (PNA) facilitates multistranded hybrid formation between linear double-stranded DNA targets and RecA protein-coated complementary single-stranded DNA probes
Belotserkovskii, BP
Zarling, DA
[J]. BIOCHEMISTRY, 2002, 41 (11) : 3686 - 3692
[7] Superior duplex DNA strand invasion by acridine conjugated peptide nucleic acids
Bentin, T
Nielsen, PE
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (21) : 6378 - 6379
[8] Enhanced peptide nucleic acid binding to supercoiled DNA: Possible implications for DNA ''breathing'' dynamics
Bentin, T
Nielsen, PE
[J]. BIOCHEMISTRY, 1996, 35 (27) : 8863 - 8869
[9] Design of a targeted peptide nucleic acid prodrug to inhibit hepatic human microsomal triglyceride transfer protein expression in hepatocytes
Biessen, EAL
Sliedregt-Bol, K
Hoen, PACT
Prince, P
Van der Bilt, E
Valentijn, ARPM
Meeuwenoord, NJ
Princen, H
Bijsterbosch, MK
Van der Marel, GA
Van Boom, JH
Van Berkel, TJC
[J]. BIOCONJUGATE CHEMISTRY, 2002, 13 (02) : 295 - 302
[10] Invasion of the CAG triplet repeats by a complementary peptide nucleic acid inhibits transcription of the androgen receptor and TATA-binding protein genes and correlates with refolding of an active nucleosome containing a unique AR gene sequence
Boffa, LC
Morris, PL
Carpaneto, EM
Louissaint, M
Allfrey, VG
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (22) : 13228 - 13233

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