Facile synthesis of a fluorescent deoxycytidine analogue suitable for probing the ReCA nucleoprotein filament

被引：36

作者：

Singleton, SF

Shan, F

Kanan, MW

McIntosh, CM

Stearman, CJ

Helm, JS

Webb, KJ

机构：

[1] Rice Univ, Dept Chem, Houston, TX 77251 USA

[2] Rice Univ, Dept Biochem & Cell Biol, Houston, TX 77251 USA

来源：

ORGANIC LETTERS | 2001年 / 3卷 / 24期

关键词：

D O I：

10.1021/ol0167863

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

equation presented We report the synthesis of the fluorescent 2′-deoxycytidine analogue 5-methylpyrimidin-2-one nucleoside, its incorporation at three specified sites in a single 60-nucleotide DNA molecule, and the use of its total and polarized intrinsic fluorescence to characterize RecA-DNA complexes.

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收藏

页码：3919 / 3922

页数：4

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