Ammonium can stimulate nitrate and nitrite reductase in the absence of nitrate in Clematis vitalba

被引:39
作者
Bungard, RA
Wingler, A
Morton, JD
Andrews, M
Press, MC
Scholes, JD
机构
[1] Univ Sheffield, Dept Anim & Plant Sci, Sheffield S10 2TN, S Yorkshire, England
[2] Lincoln Univ, Anim & Food Sci Div, Canterbury, New Zealand
[3] Univ Sunderland, Ctr Ecol, Sunderland SR1 3SD, England
关键词
Clematis vitalba; ammonium; glutamine synthetase; nitrate reductase; nitrite reductase; nitrate; nitrogen; pH;
D O I
10.1046/j.1365-3040.1999.00456.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Nitrogen assimilation was studied in the deciduous, perennial climber Clematis vitalba, When solely supplied with NO3- in a hydroponic system, growth and N-assimilation characteristics were similar to those reported for a range of other species. When solely supplied with NH4+, however, nitrate reductase (NR) activity dramatically increased in shoot tissue, and particularly leaf tissue, to up to three times the maximum level achieved in NO3- supplied plants, NO3- was not detected in plant material that had been solely supplied with NH4+, there was no NO3- contamination of the hydroponic system, and the NH4+-induced activity did not occur in tobacco or barley grown under similar conditions. Western Blot analysis revealed that the induction of NR activity, either by NO3- or NH4+, was matched by NR and nitrite reductase protein synthesis, but this was not the case for the ammonium assimilation enzyme glutamine synthetase, Exposure of leaf disks to N revealed that NO3- assimilation was induced in leaves directly by NO3- and NH4+ but not glutamine, Our results suggest that the NH4+-induced potential for NO3- assimilation occurs when externally sourced NH4+ is assimilated in the absence of any NO3- assimilation. These data show that the potential for nitrate assimilation in C. vitalba is induced by a nitrogenous compound in the absence of its substrate and suggest that NO3- assimilation in C, vitalba may have a significant role beyond the supply of reduced N for growth.
引用
收藏
页码:859 / 866
页数:8
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