Random and directed mutagenesis to elucidate the functional importance of helix II and F-989 in the C-terminal secretion signal of Escherichia coli hemolysin

被引：34

作者：

Chervaux, C ^{[1
]}

Holland, IB ^{[1
]}

机构：

[1] UNIV PARIS 11, INST MICROBIOL & GENET, F-91405 ORSAY, FRANCE

来源：

JOURNAL OF BACTERIOLOGY | 1996年 / 178卷 / 04期

关键词：

D O I：

10.1128/jb.178.4.1232-1236.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The HlyA secretion signal sequence of approximately 46 residues is predicted to contain helix I and an amphipathic helix II separated by a short loop including the conserved Phe residue, F-989. All nine substitutions of Phe-989 drastically reduce secretion of HlyA. Directed mutagenesis identified a functional hot spot, EISK, in helix II. However, genetic analysis did not provide strong support for a functional helix II; rather, the results emphasized that individual residues, for example, E-978 and F-989, are essential irrespective of a specific secondary structure.

引用

页码：1232 / 1236

页数：5

共 27 条

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