Multiplex PCR assay for direct identification of group B streptococcal alpha-protein-like protein genes

被引：110

作者：

Creti, R ^{[1
]}

Fabretti, F ^{[1
]}

Orefici, G ^{[1
]}

von Hunolstein, C ^{[1
]}

机构：

[1] Ist Super Sanita, Lab Batteriol, I-00161 Rome, Italy

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2004年 / 42卷 / 03期

关键词：

D O I：

10.1128/JCM.42.3.1326-1329.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We developed a group B streptococcus multiplex PCR assay which allows, by direct analysis of the amplicon size, determination of the surface protein antigen genes of alpha-C protein, epsilon protein, Rib, Alp2, Alp3, and Alp4. The multiplex PCR assay offers a rapid and simple method of subtyping Streptococcus agalactiae based on surface protein genes.

引用

收藏

页码：1326 / 1329

页数：4

相关论文

共 20 条

[1] Group B streptococcal conjugate vaccines [J].

Baker, CJ ;

Edwards, MS .

ARCHIVES OF DISEASE IN CHILDHOOD, 2003, 88 (05) :375-378

[2] The alpha C protein mediates internalization of group B Streptococcus within human cervical epithelial cells [J].

Bolduc, GR ;

Baron, MJ ;

Gravekamp, C ;

Lachenauer, CS ;

Madoff, LC .

CELLULAR MICROBIOLOGY, 2002, 4 (11) :751-758

[3]

BORCHARDT SM, 2003, ANN EPIDEMIOL, V13, P581

[4]

CRETI R, 2000, STREPTOCOCCI STREPTO, P397

[5]

FERRIERI P, 1988, REV INFECT DIS, V10, P363

[6] Alpha C protein as a carrier for type III capsular polysaccharide and as a protective protein in group B streptococcal vaccines [J].

Gravekamp, C ;

Kasper, DL ;

Paoletti, LC ;

Madoff, LC .

INFECTION AND IMMUNITY, 1999, 67 (05) :2491-2496

[7] MOLECULAR CHARACTERIZATION OF AN IGA RECEPTOR FROM GROUP-B STREPTOCOCCI - SEQUENCE OF THE GENE, IDENTIFICATION OF A PROLINE-RICH REGION WITH UNIQUE STRUCTURE AND ISOLATION OF N-TERMINAL FRAGMENTS WITH IGA-BINDING CAPACITY [J].

HEDEN, LO ;

FRITHZ, E ;

LINDAHL, G .

EUROPEAN JOURNAL OF IMMUNOLOGY, 1991, 21 (06) :1481-1490

[8] THE IGA-BINDING BETA-ANTIGEN OF THE C-PROTEIN COMPLEX OF GROUP-B STREPTOCOCCI - SEQUENCE DETERMINATION OF ITS GENE AND DETECTION OF 2 BINDING REGIONS [J].

JERLSTROM, PG ;

CHHATWAL, GS ;

TIMMIS, KN .

MOLECULAR MICROBIOLOGY, 1991, 5 (04) :843-849

[9] Molecular profiles of group B streptococcal surface protein antigen genes: Relationship to molecular serotypes [J].

Kong, FR ;

Gowan, S ;

Martin, D ;

James, G ;

Gilbert, GL .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (02) :620-626

[10] Serotype identification of group B streptococci by PCR and sequencing [J].

Kong, FR ;

Gowan, S ;

Martin, D ;

James, G ;

Gilbert, GL .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (01) :216-226