The anti-inflammatory effect of alloferon on UVB-induced skin inflammation through the down-regulation of pro-inflammatory cytokines

被引:35
作者
Kim, Yejin [1 ]
Lee, Seung Koo [1 ]
Bae, Seyeon [1 ]
Kim, Hyemin [1 ]
Park, Yunseong [2 ]
Chu, Nag Kyun [3 ]
Kim, Stephanie G. [3 ]
Kim, Hang-Rae [1 ]
Hwang, Young-il [1 ]
Kang, Jae Seung [1 ,4 ]
Lee, Wang Jae [1 ]
机构
[1] Seoul Natl Univ, Coll Med, Dept Anat, Seoul 110799, South Korea
[2] Seoul Natl Univ, Coll Med, Dept Med, Seoul 110799, South Korea
[3] Univ Wisconsin Madison, Dept Biol, Madison, WI USA
[4] Seoul Natl Univ, Med Res Ctr, Inst Complementary & Integrat Med, Seoul 110799, South Korea
关键词
Alloferon; Interleukin-6; p38 mitogen-activated protein kinase; Inflammation; Ultraviolet B radiation; SIGNAL-TRANSDUCTION PATHWAYS; NF-KAPPA-B; IN-VIVO; C-JUN; EPIDERMAL-KERATINOCYTES; ULTRAVIOLET-RADIATION; GENE-EXPRESSION; MAP KINASE; CELL-LINE; PROTEIN;
D O I
10.1016/j.imlet.2012.09.005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
UVB irradiation can induce biological changes in the skin, modulate immune responses and activate inflammatory reactions leading to skin damage. Alloferon, which is isolated from the blood of an experimentally infected insect, the blow fly Calliphora vicina, is known for its anti-viral and anti-tumor activities in mice model. However, the effect of alloferon against UVB irradiation and its specific mechanism are still unknown. In this study, we investigated the effect of alloferon on UVB-induced cutaneous inflammation in a human keratinocyte cell line, HaCaT. RPA and ELISA data showed that alloferon decreased the production of UVB-induced pro-inflammatory cytokines, such as IL-1 alpha, IL-1 beta, IL-6 and IL-18, both on the mRNA and protein level. Western blot analysis was done to determine if alloferon regulates the MAPK signaling pathway since the MAPK signaling pathway is activated by numerous inflammatory mediators and environmental stresses including UVB irradiation. Alloferon inhibited the activation of p38 mitogen-activated protein kinase (MAPK) induced by UVB irradiation. Furthermore, the topical application of alloferon on the UVB exposed skin of hairless mice showed that alloferon treatment significantly inhibited an increase in epithelial thickness in chronic UVB-irradiated mouse skin. These findings suggest that alloferon has significant anti-inflammatory effects not only on UVB-induced inflammation in the human keratinocyte cell line, HaCaT, but also on mouse skin. (C) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:110 / 118
页数:9
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