Smart latexes for bioseparation

被引：103

作者：

Kawaguchi, H ^{[1
]}

Fujimoto, K ^{[1
]}

机构：

[1] Keio Univ, Fac Sci & Technol, Dept Appl Chem, Yokohama, Kanagawa 2238522, Japan

来源：

BIOSEPARATION | 1998年 / 7卷 / 4-5期

关键词：

coagulation; hydrophilicity; latex; microsphere; thermosensitive polymer;

D O I：

10.1023/A:1008055211667

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Monodisperse, thermosensitive microspheres with sub-micron diameters are used for separation and collection of proteins and other biomolecules. The thermosensitivity gives the microspheres two valuable features. One is the controllability of affinity between microsphere and protein with temperature. The quality and quantity of proteins to be adsorbed on the microspheres can be controlled with temperature. The other feature is reversible control of dispersion stability. Microspheres which have adsorbed target proteins in the dispersion can be easily recovered by changing temperature.

引用

页码：253 / 258

页数：6

共 25 条

[1]

DING Z, 1998, BIOCONJUGATE CHEM, V9, P498

[2] FLUORESCENCE ANALYSIS FOR THERMOSENSITIVE HYDROGEL MICROSPHERES [J].

FUJIMOTO, K ;

NAKAJIMA, Y ;

KASHIWABARA, M ;

KAWAGUCHI, H .

POLYMER INTERNATIONAL, 1993, 30 (02) :237-241

[3]

Fujimoto K, 1993, J INTEL MAT SYST STR, V4, P184, DOI DOI 10.1177/1045389X9300400207

[4]

FUJIMOTO K, 1997, KAGAKU TO KOGYO, V50, P1523

[5] GLOBULAR-PROTEINS AT SOLID-LIQUID INTERFACES [J].

HAYNES, CA ;

NORDE, W .

COLLOIDS AND SURFACES B-BIOINTERFACES, 1994, 2 (06) :517-566

[6] N-SUBSTITUTED ACRYLAMIDE STYRENE COPOLYMER LATTICES .2. POLYMERIZATION BEHAVIOR AND THERMOSENSITIVE STABILITY OF LATTICES [J].

HOSHINO, F ;

FUJIMOTO, T ;

KAWAGUCHI, H ;

OHTSUKA, Y .

POLYMER JOURNAL, 1987, 19 (02) :241-247

[7] SOAP-FREE LATTICES OF POLYOXYETHYLENE CHAIN-BINDING PARTICLES [J].

HOSHINO, F ;

SAKAI, M ;

KAWAGUCHI, H ;

OHTSUKA, Y .

POLYMER JOURNAL, 1987, 19 (04) :383-389

[8] N-SUBSTITUTED ACRYLAMIDE STYRENE COPOLYMER LATTICES .3. MORPHOLOGY OF LATEX-PARTICLES [J].

HOSHINO, F ;

KAWAGUCHI, H ;

OHTSUKA, Y .

POLYMER JOURNAL, 1987, 19 (10) :1157-1164

[9] Temperature-responsive liquid chromatography .2. Effects of hydrophobic groups in N-isopropylacrylamide copolymer-modified silica [J].

Kanazawa, H ;

Kashiwase, Y ;

Yamamoto, K ;

Matsushima, Y ;

Kikuchi, A ;

Sakurai, Y ;

Okano, T .

ANALYTICAL CHEMISTRY, 1997, 69 (05) :823-830

[10] PREPARATION OF MONODISPERSE, REACTIVE HYDROGEL MICROSPHERES AND THEIR AMPHOTERIZATION [J].

KASHIWABARA, M ;

FUJIMOTO, K ;

KAWAGUCHI, H .

COLLOID AND POLYMER SCIENCE, 1995, 273 (04) :339-345

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