Microdetermination of proteins by resonance light scattering spectroscopy with bromophenol blue

被引：95

作者：

Ma, CQ ^{[1
]}

Li, KA ^{[1
]}

Tong, SY ^{[1
]}

机构：

[1] BEIJING UNIV, DEPT CHEM, BEIJING 100871, PEOPLES R CHINA

来源：

ANALYTICAL BIOCHEMISTRY | 1996年 / 239卷 / 01期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1006/abio.1996.0294

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Reaction of bromophenol blue with proteins results in an enhanced resonance light scattering at 334 nm. Based on this, a new quantitative determination method for proteins in the aqueous solution is established. This assay is characterized by high sensitivity (0.34-18.7 mu g/ml), short reaction time (>2 min), and simplicity (a one-step assay). Due to protein-to-protein variability, this method gave results higher than that of the bromocresol green assay in detection of human serum albumin. (C) 1996 Academic Press, Inc.

引用

页码：86 / 91

页数：6

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