A Genetically Encoded Boronate-Containing Amino Acid

被引：131

作者：

Brustad, Eric

Bushey, Mark L.

Lee, Jae Wook

Groff, Dan

Liu, Wenshe

Schultz, Peter G. ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 43期

关键词：

affinity purification; boronic acids; nonnatural amino acids; protein modification;

D O I：

10.1002/anie.200803240

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) A biological boronate: An orthogonal tRNA/aminoacyl-tRNA synthetase pair has been evolved for the genetic incorporation of a boronic acid into proteins. This amino acid has been used to purify proteins in a one-step scarless purification procedure as well as for the site-specific labeling of proteins using various boronic acid chemistries. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：8220 / 8223

页数：4

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