Structures of diphospho-myo-inositol pentakisphosphate and bisdiphospho-myo-inositol tetrakisphosphate from Dictyostelium resolved by NMR analysis

被引：50

作者：

Laussmann, T ^{[1
]}

Eujen, R ^{[1
]}

Weisshuhn, CM ^{[1
]}

Thiel, U ^{[1
]}

Vogel, G ^{[1
]}

机构：

[1] BERG UNIV GESAMTHSCH WUPPERTAL,FACHBEREICH CHEM 9,D-42097 WUPPERTAL,GERMANY

来源：

BIOCHEMICAL JOURNAL | 1996年 / 315卷

关键词：

D O I：

10.1042/bj3150715

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Diphospho-myo-inositol phosphates (PP-InsP(5) and bis-PP-InsP(4)) were isolated from Dictyostelium in order to clarify the precise positional isomerism by two-dimensional H-1/P-31-NMR analysis. The diphosphorylated inositol phosphates are 4-PP-Ins(1,2,3,5,6)P-5 and 4,5-bis-PP-Ins(1,2,3,6)P-4 or their corresponding enantiomers. The vicinal arrangement of the diphospho groups with its steric and electrostatic constraints possibly qualifies bis-PP-InsP(4) as a metabolite with high phosphate-group-transfer potential in phosphotransferase reactions.

引用

页码：715 / 720

页数：6

共 17 条

[1] 2-DIMENSIONAL SPECTROSCOPY - APPLICATION TO NUCLEAR MAGNETIC-RESONANCE [J].

AUE, WP ;

BARTHOLDI, E ;

ERNST, RR .

JOURNAL OF CHEMICAL PHYSICS, 1976, 64 (05) :2229-2246

[2] AN IMPROVED METHOD FOR HETERONUCLEAR CHEMICAL-SHIFT CORRELATION BY TWO-DIMENSIONAL NMR [J].

BAX, A ;

MORRIS, GA .

JOURNAL OF MAGNETIC RESONANCE, 1981, 42 (03) :501-505

[3] SYNTHESIS AND STRUCTURE OF CELLULAR MEDIATORS - INOSITOL POLYPHOSPHATE DIPHOSPHATES [J].

FALCK, JR ;

REDDY, KK ;

YE, JH ;

SAADY, M ;

MIOSKOWSKI, C ;

SHEARS, SB ;

TAN, Z ;

SAFRANY, S .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1995, 117 (49) :12172-12175

[4]

FLEISCHER B, 1994, J BIOL CHEM, V269, P17826

[5] TURNOVER OF INOSITOL PENTAKISPHOSPHATES, INOSITOL HEXAKISPHOSPHATE AND DIPHOSPHOINOSITOL POLYPHOSPHATES IN PRIMARY CULTURED-HEPATOCYTES [J].

GLENNON, MC ;

SHEARS, SB .

BIOCHEMICAL JOURNAL, 1993, 293 :583-590

[6] APPLICATION OF 2-DIMENSIONAL TOTAL CORRELATION SPECTROSCOPY FOR STRUCTURE DETERMINATION OF INDIVIDUAL INOSITOL PHOSPHATES IN A MIXTURE [J].

JOHNSON, K ;

BARRIENTOS, LG ;

LE, L ;

MURTHY, PPN .

ANALYTICAL BIOCHEMISTRY, 1995, 231 (02) :421-431

[7] METABOLITES OF THE FREE-LIVING AMEBA PHREATAMOEBA-BALAMUTHI ANALYZED BY C-13-NMR AND P-31-NMR SPECTROSCOPY - OCCURRENCE OF PHOSPHOINOSITOL DIPHOSPHATES [J].

MARTIN, JB ;

BAKKERGRUNWALD, T ;

KLEIN, G .

JOURNAL OF EUKARYOTIC MICROBIOLOGY, 1995, 42 (02) :183-191

[8] P-31-NMR ANALYSIS OF ENTAMOEBA-HISTOLYTICA - OCCURRENCE OF HIGH AMOUNTS OF 2 INOSITOL PHOSPHATES [J].

MARTIN, JB ;

BAKKERGRUNWALD, T ;

KLEIN, G .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 214 (03) :711-718

[9] PHOSPHOINOSITOL DIPHOSPHATES - NONENZYMATIC FORMATION INVITRO AND OCCURRENCE INVIVO IN THE CELLULAR SLIME-MOLD DICTYOSTELIUM [J].

MAYR, GW ;

RADENBERG, T ;

THIEL, U ;

VOGEL, G ;

STEPHENS, LR .

CARBOHYDRATE RESEARCH, 1992, 234 :247-262

[10] A NOVEL METAL-DYE DETECTION SYSTEM PERMITS PICOMOLAR-RANGE HPLC ANALYSIS OF INOSITOL POLYPHOSPHATES FROM NON-RADIOACTIVELY LABELED CELL OR TISSUE SPECIMENS [J].

MAYR, GW .

BIOCHEMICAL JOURNAL, 1988, 254 (02) :585-591

← 1 2 →