cDNA cloning and characterization of tobacco ABC transporter:: NtPDR1 is a novel elicitor-responsive gene

被引：71

作者：

Sasabe, M ^{[1
]}

Toyoda, K ^{[1
]}

Shiraishi, T ^{[1
]}

Inagaki, Y ^{[1
]}

Ichinose, Y ^{[1
]}

机构：

[1] Okayama Univ, Lab Plant Pathol & Genet Engn, Fac Agr, Okayama 7008530, Japan

来源：

FEBS LETTERS | 2002年 / 518卷 / 1-3期

基金：

日本学术振兴会;

关键词：

ATP-binding cassette transporter; tobacco bright yellow-2; elicitin; elicitor; defense response;

D O I：

10.1016/S0014-5793(02)02697-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated an INF1 elicitin-inducible cDNA encoding a pleiotropic drug resistance (PDR)-type ATP-binding cassette (A-BC) transporter homolog (NtPDR1) in suspension-cultured tobacco Bright Yellow-2 (BY-2) cells by application of differential display PCR. The NtPDR1 (Nicotiana tabacum PDR protein 1) gene also encodes a 162 kDa protein that includes two putative hydrophilic domains containing the ABC signature motif and two putative hydrophobic domains. Expression of the NtPDR1 gene was rapidly and strongly activated by treatment of BY-2 cells with INF1 elicitin. Further, treatment of BY-2 cells with flagellin, a bacterial proteinaceous hypersensitive reaction elicitor, or yeast extract, a general elicitor, also induced NtPDR1 gene expression. These results indicate that NtPDR1 may be involved in the general defense response in tobacco. This is the first report that microbial elicitors induce the expression of a plant ABC transporter gene. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：164 / 168

页数：5

共 33 条

[1] The InterPro database, an integrated documentation resource for protein families, domains and functional sites [J].

Apweiler, R ;

Attwood, TK ;

Bairoch, A ;

Bateman, A ;

Birney, E ;

Biswas, M ;

Bucher, P ;

Cerutti, T ;

Corpet, F ;

Croning, MDR ;

Durbin, R ;

Falquet, L ;

Fleischmann, W ;

Gouzy, J ;

Hermjakob, H ;

Hulo, N ;

Jonassen, I ;

Kahn, D ;

Kanapin, A ;

Karavidopoulou, Y ;

Lopez, R ;

Marx, B ;

Mulder, NJ ;

Oinn, TM ;

Pagni, M ;

Servant, F ;

Sigrist, CJA ;

Zdobnov, EM .

NUCLEIC ACIDS RESEARCH, 2001, 29 (01) :37-40

[2] Biosynthesis and action of jasmonates in plants [J].

Creelman, RA ;

Mullet, JE .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1997, 48 :355-381

[3] MOLECULAR COMMUNICATION IN INTERACTIONS BETWEEN PLANTS AND MICROBIAL PATHOGENS [J].

DIXON, RA ;

LAMB, CJ .

ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 1990, 41 :339-367

[4] Induction of tcI 7, a gene encoding a β-subunit of proteasome, in tobacco plants treated with elicitins, salicylic acid or hydrogen peroxide [J].

Etienne, P ;

Petitot, AS ;

Houot, V ;

Blein, JP ;

Suty, L .

FEBS LETTERS, 2000, 466 (2-3) :213-218

[5] JASMONIC ACID IS A SIGNAL TRANSDUCER IN ELICITOR-INDUCED PLANT-CELL CULTURES [J].

GUNDLACH, H ;

MULLER, MJ ;

KUTCHAN, TM ;

ZENK, MH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (06) :2389-2393

[6] ABC TRANSPORTERS - FROM MICROORGANISMS TO MAN [J].

HIGGINS, CF .

ANNUAL REVIEW OF CELL BIOLOGY, 1992, 8 :67-113

[7] BINDING PROTEIN-DEPENDENT TRANSPORT-SYSTEMS [J].

HIGGINS, CF ;

HYDE, SC ;

MIMMACK, MM ;

GILEADI, U ;

GILL, DR ;

GALLAGHER, MP .

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1990, 22 (04) :571-592

[8] Crystal structure of the ATP-binding subunit of an ABC transporter [J].

Hung, LW ;

Wang, IXY ;

Nikaido, K ;

Liu, PQ ;

Ames, GFL ;

Kim, SH .

NATURE, 1998, 396 (6712) :703-707

[9]

Ichinose Y, 2000, Z NATURFORSCH C, V55, P44

[10] A plant plasma membrane ATP binding cassette-type transporter is involved in antifungal terpenoid secretion [J].

Jasinski, M ;

Stukkens, Y ;

Degand, H ;

Purnelle, B ;

Marchand-Brynaert, J ;

Boutry, M .

PLANT CELL, 2001, 13 (05) :1095-1107

← 1 2 3 4 →