cDNA cloning and characterization of tobacco ABC transporter:: NtPDR1 is a novel elicitor-responsive gene

被引:71
作者
Sasabe, M [1 ]
Toyoda, K [1 ]
Shiraishi, T [1 ]
Inagaki, Y [1 ]
Ichinose, Y [1 ]
机构
[1] Okayama Univ, Lab Plant Pathol & Genet Engn, Fac Agr, Okayama 7008530, Japan
基金
日本学术振兴会;
关键词
ATP-binding cassette transporter; tobacco bright yellow-2; elicitin; elicitor; defense response;
D O I
10.1016/S0014-5793(02)02697-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We isolated an INF1 elicitin-inducible cDNA encoding a pleiotropic drug resistance (PDR)-type ATP-binding cassette (A-BC) transporter homolog (NtPDR1) in suspension-cultured tobacco Bright Yellow-2 (BY-2) cells by application of differential display PCR. The NtPDR1 (Nicotiana tabacum PDR protein 1) gene also encodes a 162 kDa protein that includes two putative hydrophilic domains containing the ABC signature motif and two putative hydrophobic domains. Expression of the NtPDR1 gene was rapidly and strongly activated by treatment of BY-2 cells with INF1 elicitin. Further, treatment of BY-2 cells with flagellin, a bacterial proteinaceous hypersensitive reaction elicitor, or yeast extract, a general elicitor, also induced NtPDR1 gene expression. These results indicate that NtPDR1 may be involved in the general defense response in tobacco. This is the first report that microbial elicitors induce the expression of a plant ABC transporter gene. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
引用
收藏
页码:164 / 168
页数:5
相关论文
共 33 条
[21]   The ABC transporter BcatrB affects the sensitivity of Botrytis cinerea to the phytoalexin resveratrol and the fungicide fenpiclonil [J].
Schoonbeek, H ;
Del Sorbo, G ;
De Waard, MA .
MOLECULAR PLANT-MICROBE INTERACTIONS, 2001, 14 (04) :562-571
[22]   The catalytic cycle of P-glycoprotein [J].
Senior, AE ;
AlShawi, MK ;
Urbatsch, IL .
FEBS LETTERS, 1995, 377 (03) :285-289
[23]   Thr38 and Ser198 are Pto autophosphorylation sites required for the AvrPto-Pto-mediated hypersensitive response [J].
Sessa, G ;
D'Ascenzo, M ;
Martin, GB .
EMBO JOURNAL, 2000, 19 (10) :2257-2269
[24]   Hormonal and environmental regulation of a plant PDR5-like ABC transporter [J].
Smart, CC ;
Fleming, AJ .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (32) :19351-19357
[25]  
TAGUCHI F, 2002, UNPUB
[26]   CLUSTAL-W - IMPROVING THE SENSITIVITY OF PROGRESSIVE MULTIPLE SEQUENCE ALIGNMENT THROUGH SEQUENCE WEIGHTING, POSITION-SPECIFIC GAP PENALTIES AND WEIGHT MATRIX CHOICE [J].
THOMPSON, JD ;
HIGGINS, DG ;
GIBSON, TJ .
NUCLEIC ACIDS RESEARCH, 1994, 22 (22) :4673-4680
[27]   An ABC-transporter of Arabidopsis thaliana has both glutathione-conjugate and chlorophyll catabolite transport activity [J].
Tommasini, R ;
Vogt, E ;
Fromenteau, M ;
Hörtensteiner, S ;
Matile, P ;
Amrhein, N ;
Martinoia, E .
PLANT JOURNAL, 1998, 13 (06) :773-780
[28]   Differential expression of genes coding for ABC transporters after treatment of Arabidopsis thaliana with xenobiotics [J].
Tommasini, R ;
Vogt, E ;
Schmid, J ;
Fromentau, M ;
Amrhein, N ;
Martinoia, E .
FEBS LETTERS, 1997, 411 (2-3) :206-210
[29]   An ATP-driven efflux pump is a novel pathogenicity factor in rice blast disease [J].
Urban, M ;
Bhargava, T ;
Hamer, JE .
EMBO JOURNAL, 1999, 18 (03) :512-521
[30]   DISTANTLY RELATED SEQUENCES IN THE ALPHA-SUBUNITS AND BETA-SUBUNITS OF ATP SYNTHASE, MYOSIN, KINASES AND OTHER ATP-REQUIRING ENZYMES AND A COMMON NUCLEOTIDE BINDING FOLD [J].
WALKER, JE ;
SARASTE, M ;
RUNSWICK, MJ ;
GAY, NJ .
EMBO JOURNAL, 1982, 1 (08) :945-951