Increased micronucleus frequency in the progeny of irradiated Chinese hamster cells

被引:38
作者
Jamali, M [1 ]
Trott, KR [1 ]
机构
[1] ST BARTHOLOMEWS HOSP,COLL MED,DEPT RADIAT BIOL,LONDON EC1M 6BQ,ENGLAND
关键词
D O I
10.1080/095530096145850
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
V79 Chinese hamster cells were irradiated with doses of 1-12 Gy 300 kV X-rays. Their colony-forming ability and the frequency of micronuclei in binucleate cells after treatment with cytochalasin B was determined at various times after irradiation. The frequency of micronuclei determined within the first 24 h after irradiation increased with doses up to 4 Gy and decreased as the dose increased further. Up to 4 Gy there was a close correlation between surviving fraction and the fraction of cells without micronuclei although the surviving fraction was 2-3 times lower than the fraction of cells without micronuclei. Six, 10 or 13 days after irradiation with either 9 or 12 Gy the plating efficiency and the frequency of micronuclei after cytokinesis block with cytochalasin B was deter mined in the irradiated, but surviving, cells. The delayed plating efficiency of irradiated cells was significantly decreased. The proportion of bi-nucleated cells was in the normal range at 6-13 days after irradiation, indicating that the proliferative activity of irradiated but surviving cells was not depressed at that time. The micronucleus frequency, however, was significantly increased at all times after irradiation. There was little heterogeneity of plating efficiency and micronucleus frequency among 12 clones which had been isolated for irradiated cultures, 3 weeks after 12 Gy.
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页码:301 / 307
页数:7
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