Analysis of the cell fusion activities of chimeric simian immunodeficiency virus murine leukemia virus envelope proteins: Inhibitory effects of the R peptide
It was previously reported that truncation or proteolytic removal of the C-terminal 16 amino acids (the R peptide) from the cytoplasmic tail of the murine leukemia virus (MuLV) envelope protein greatly increases its fusion activity, In this study, to investigate the specificity of the effect of the R peptide on the fusion activity of viral envelope proteins, we expressed simian immunodeficiency virus (SN)-MuLV chimeric proteins in which the entire cytoplasmic tail of the SIV envelope protein was replaced by either the full-length MuLV cytoplasmic tail or a truncated MuLV cytoplasmic tail with the R peptide deleted. Extensive fusion of CD4-positive cells with the chimeric protein containing a truncated MuLV cytoplasmic tail was observed. In contrast, no cell fusion activity was found for the chimeric protein,vith a full-length MuLV cytoplasmic tail. We constructed another SIV-MuLV chimeric protein in which the MuLV R peptide was added to an SIV envelope protein cytoplasmic tail 17 amino acids from its membrane-spanning domain. No fusion activity was observed within this construct, while the corresponding truncated SIV envelope protein lacking the R peptide showed extensive fusion activity, No significant difference in the transport or surface expression was observed among the various SIV-MuLV chimeric proteins and the truncated SN envelope protein. Our results thus demonstrate that the MuLV R peptide has profound inhibitory effects on virus-induced cell fusion, not only with MuLV but also in a distantly related retroviral envelope protein which utilizes a different receptor and fuses different cell types.
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NCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USANCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USA
REIN, A
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MIRRO, J
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NCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USANCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USA
MIRRO, J
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HAYNES, JG
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NCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USANCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USA
HAYNES, JG
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ERNST, SM
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NCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USANCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USA
ERNST, SM
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NAGASHIMA, K
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NCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USANCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USA
机构:
NCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USANCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USA
REIN, A
;
MIRRO, J
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机构:
NCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USANCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USA
MIRRO, J
;
HAYNES, JG
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NCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USANCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USA
HAYNES, JG
;
ERNST, SM
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机构:
NCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USANCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USA
ERNST, SM
;
NAGASHIMA, K
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机构:
NCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USANCI, FREDERICK CANC RES & DEV CTR, PROGRAM RESOURCES INC DYNCORP, CELL & MOLEC STRUCT LAB, FREDERICK, MD 21702 USA