Site-Directed Mutagenesis Reveals Putative Substrate Binding Residues in the Escherichia coli RND Efflux Pump AcrB

被引：106

作者：

Bohnert, Juergen A. ^{[1
,2
]}

Schuster, Sabine ^{[1
,2
]}

Seeger, Markus A. ^{[3
,4
]}

Faehnrich, Eva ^{[1
,2
]}

Pos, Klaas M. ^{[3
,4
]}

Kern, Winfried V. ^{[1
,2
]}

机构：

[1] Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany

[2] Univ Freiburg, Dept Med, D-7800 Freiburg, Germany

[3] Univ Zurich, Inst Physiol, Zurich, Switzerland

[4] Univ Zurich, Zurich Ctr Integrat Human Physiol, Zurich, Switzerland

来源：

JOURNAL OF BACTERIOLOGY | 2008年 / 190卷 / 24期

关键词：

D O I：

10.1128/JB.00912-08

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The Escherichia coli multidrug efflux pump protein AcrB has recently been cocrystallized with various substrates, suggesting that there is a phenylalanine-rich binding site around F178 and F615. We found that F610A was the point mutation that had the most significant impact on substrate MICs, while other targeted mutations, including conversion of phenylalanines 136, 178, 615, 617, and 628 to alanine, had smaller and more variable effects.

引用

页码：8225 / 8229

页数：5

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