共 13 条
Site-Directed Mutagenesis Reveals Putative Substrate Binding Residues in the Escherichia coli RND Efflux Pump AcrB
被引:106
作者:

Bohnert, Juergen A.
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机构:
Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany
Univ Freiburg, Dept Med, D-7800 Freiburg, Germany Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany

Schuster, Sabine
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Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany
Univ Freiburg, Dept Med, D-7800 Freiburg, Germany Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany

Seeger, Markus A.
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机构:
Univ Zurich, Inst Physiol, Zurich, Switzerland
Univ Zurich, Zurich Ctr Integrat Human Physiol, Zurich, Switzerland Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany

Faehnrich, Eva
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Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany
Univ Freiburg, Dept Med, D-7800 Freiburg, Germany Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany

Pos, Klaas M.
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Univ Zurich, Inst Physiol, Zurich, Switzerland
Univ Zurich, Zurich Ctr Integrat Human Physiol, Zurich, Switzerland Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany

Kern, Winfried V.
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机构:
Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany
Univ Freiburg, Dept Med, D-7800 Freiburg, Germany Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany
机构:
[1] Univ Hosp, Ctr Infect Dis & Travel Med, Freiburg, Germany
[2] Univ Freiburg, Dept Med, D-7800 Freiburg, Germany
[3] Univ Zurich, Inst Physiol, Zurich, Switzerland
[4] Univ Zurich, Zurich Ctr Integrat Human Physiol, Zurich, Switzerland
关键词:
D O I:
10.1128/JB.00912-08
中图分类号:
Q93 [微生物学];
学科分类号:
071005 ;
100705 ;
摘要:
The Escherichia coli multidrug efflux pump protein AcrB has recently been cocrystallized with various substrates, suggesting that there is a phenylalanine-rich binding site around F178 and F615. We found that F610A was the point mutation that had the most significant impact on substrate MICs, while other targeted mutations, including conversion of phenylalanines 136, 178, 615, 617, and 628 to alanine, had smaller and more variable effects.
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页码:8225 / 8229
页数:5
相关论文
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