Effect of aluminium ions on liposomal membranes as detected by Laurdan fluorescence

被引：11

作者：

Dousset, N ^{[1
]}

Ferreti, G ^{[1
]}

Galeazzi, T ^{[1
]}

Taus, M ^{[1
]}

Gouaze, V ^{[1
]}

Berthon, G ^{[1
]}

Curatola, G ^{[1
]}

机构：

[1] FAC MED & CHIRURG,IST BIOCHIM,I-60100 ANCONA,ITALY

来源：

FREE RADICAL RESEARCH | 1997年 / 27卷 / 03期

关键词：

aluminium; membranes; oxidation; Laurdan; fluorescence; spectra; generalised polarisation;

D O I：

10.3109/10715769709065767

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report here an investigation of the influence of aluminium on iron-induced peroxidation in brain model membranes. Laurdan fluorescence emission spectra and generalised polarisation measurements have been used to investigate how ferrous and aluminium ions can affect the phase components of phospholipid membranes. An increase in the generalised polarisation of oxidised liposomes with respect to controls has been observed, which reveals the presence of a less polar environment surrounding the probe that changes the properties of the bilayer. Aluminium has been shown to facilitate iron-mediated oxidation as detected from emission fluorescence spectra. However, no quantitative influence has been calculated relative to general polarisation and derived phase state determinations. The structural influence of aluminium on membranes may therefore be less significantly marked than initially expected.

引用

页码：291 / 299

页数：9

共 36 条

[1]

ARAI H, 1987, BIOCH INT, V14, P714

[2] HYDROGEN-PEROXIDE MEDIATES AMYLOID-BETA PROTEIN TOXICITY [J].