Human embryonic stem cells have enhanced repair of multiple forms of DNA damage

被引：158

作者：

Maynard, Scott ^{[1
]}

Swistowska, Anna Maria ^{[2
]}

Lee, Jae Wan ^{[3
]}

Liu, Ying ^{[4
]}

Liu, Su-Ting ^{[1
]}

Da Cruz, Alexandre Bettencourt ^{[2
]}

Rao, Mahendra ^{[4
]}

de Souza-Pinto, Nadja C. ^{[1
]}

Zeng, Xianmin ^{[2
]}

Bohr, Vilhelm A. ^{[1
]}

机构：

[1] NIA, Lab Mol Gerontol, NIH, Baltimore, MD 21224 USA

[2] Buck Inst Age Res, Novato, CA USA

[3] US Patent & Trademark Off, Crystallog & Recombinant Enzyme Art Unit, Alexandria, VA USA

[4] Invitrogen Corp, Carlsbad, CA USA

来源：

STEM CELLS | 2008年 / 26卷 / 09期

关键词：

human embryonic stem cells; DNA repair; genomic maintenance; comet assay; microarray;

D O I：

10.1634/stemcells.2007-1041

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Embryonic stem cells need to maintain genomic integrity so that they can retain the ability to differentiate into multiple cell types without propagating DNA errors. Previous studies have suggested that mechanisms of genome surveillance, including DNA repair, are superior in mouse embryonic stem cells compared with various differentiated murine cells. Using single-cell gel electrophoresis (comet assay) we found that human embryonic stem cells (BG01, I6) have more efficient repair of different types of DNA damage (generated from H2O2, UV-C, ionizing radiation, or psoralen) than human primary fibroblasts (WI-38, hs27) and, with the exception of UV-C damage, HeLa cells. Microarray gene expression analysis showed that mRNA levels of several DNA repair genes are elevated in human embryonic stem cells compared with their differentiated forms (embryoid bodies). These data suggest that genomic maintenance pathways are enhanced in human embryonic stem cells, relative to differentiated human cells.

引用

页码：2266 / 2274

页数：9

共 52 条

[11]

Green MHL, 1996, METHOD ENZYMOL, V269, P243

[12] Protecting genomic integrity in somatic cells and embryonic stem cells [J].