Selective Extraction of Bacterial DNA from the Surfaces of Macroalgae

被引：48

作者：

Burke, Catherine

Kjelleberg, Staffan ^{[1
]}

Thomas, Torsten

机构：

[1] Univ New S Wales, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 01期

基金：

澳大利亚研究理事会;

关键词：

MARINE-BACTERIA; DIVERSITY; COMMUNITY; ALGAE; SEAWEEDS; ANTIBACTERIAL; COLONIZATION; EUKARYOTES; ATTACHMENT; GROWTH;

D O I：

10.1128/AEM.01630-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel method has been developed for the selective extraction of DNA from surface-associated bacterial communities from the two model marine benthic algae Ulva australis and Delisea pulchra. The extracted DNA had no detectable contamination with host DNA, was recovered in high yield and quality, and was representative of the bacterial community on the algal surfaces. The DNA is suitable for a variety of subsequent applications, including the construction of large-insert clone libraries and metagenomic sequencing.

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收藏

页码：252 / 256

页数：5

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