Trends in metal-binding and metalloprotein analysis

被引：123

作者：

Garcia, JS ^{[1
]}

de Magalhaes, CS ^{[1
]}

Arruda, MAZ ^{[1
]}

机构：

[1] Univ Estadual Campinas, Inst Chem, Dept Analyt Chem, BR-13084971 Campinas, SP, Brazil

来源：

TALANTA | 2006年 / 69卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

metal-binding protein; metalloprotein; separation techniques; determination techniques;

D O I：

10.1016/j.talanta.2005.08.041

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This review describes recent tendencies for metal-binding and metalloprotein analysis, emphasizing metal quantification in proteins through X-ray, atomic absorption, mass spectrometric techniques, and others. Hyphenated techniques such as capillary electrophoresis-synchrotron radiation X-ray fluorescence (CE-SRXRF), laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS), matrix-assisted laser desorption/ionisation-time-of-flight mass spectrometry (MALDI-TOF-MS), etc. are also presented. As protein separation techniques electrophoresis (mainly sodium dodecyl sulphate-polyacrylamide gel electrophoresis, SDS-PAGE), capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC) are indicated, due to their inherent sensitivity, resolution and/or easy implementation. Latest challenges in metallomics are also commented. (c) 2005 Elsevier B.V. All rights reserved.

引用

页码：1 / 15

页数：15

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