Analysis of phenols in pharmaceuticals by liquid chromatography after pre-column labelling and on-line post-column photochemical derivatization

被引：33

作者：

Gatti, R ^{[1
]}

Gioia, MG ^{[1
]}

Di Pietra, AM ^{[1
]}

Cavrini, V ^{[1
]}

机构：

[1] Univ Bologna, Dipartimento Sci Farmaceut, I-40126 Bologna, Italy

来源：

ANALYTICA CHIMICA ACTA | 2001年 / 447卷 / 1-2期

关键词：

RP-HPLC; 1-fluoro-2,4-dinitrobenzene; photochemical derivatization; phenols;

D O I：

10.1016/S0003-2670(01)01292-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Reversed phase high-performance liquid chromatographic (RP-HPLC) methods are proposed for the analysis of phenol, thymol, chlorocresol and chloroxylenol in commercial pharmaceutical dosage forms. The use of 1-fluoro-2,4-dinitrobenzene as UV labelling reagent in pre-column derivatization has been investigated. The compound reacts rapidly (2 min) under mild conditions (ambient temperature or 40 degreesC) with phenols to give ethers that can be separated by RP-HPLC and detected at 292 nm. The other procedure involves a post-column on-line photochemical conversion. A photoreactor was arranged between the analytical column and the fluorescence (lambda (ex) = 270 nm, lambda (em) = 310 mn) and UV-diode array (lambda = 270 nm) detector to enhance the performance of the methods. Additional informations of the analyte structure and photoreactivity by UV spectra (photoreactor 'on' and 'off) were obtained. The methods showed good selectivity and sensitivity. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：89 / 99

页数：11

共 18 条

[1] SEMI-AUTOMATED KINETIC DETERMINATION OF PHENOLIC-COMPOUNDS USING A FLUORIDE-SELECTIVE ELECTRODE AND BASED ON THEIR MICELLAR-CATALYZED REACTION WITH 1-FLUORO-2,4-DINITROBENZENE [J].

ARCHONTAKI, HA ;

KOUPPARIS, MA ;

EFSTATHIOU, CE .

ANALYST, 1989, 114 (05) :591-596

[2]

BLAU K, 1993, HDB DERIVATIVES CHRO, P161

[3] MICELLAR CATALYSIS OF REACTION OF 2,4-DINITROFLUOROBENZENE WITH PHENOXIDE AND THIOPHENOXIDE IONS [J].

CHAIMOVICH, H ;

BLANCO, A ;

CHAYET, L ;

COSTA, LM ;

MONTEIRO, PM ;

BUNTON, CA ;

PAIK, C .

TETRAHEDRON, 1975, 31 (09) :1139-1143

[4] ENHANCED FLUORESCENCE DETECTION OF DANSYL DERIVATIVES OF PHENOLIC-COMPOUNDS USING A POSTCOLUMN PHOTOCHEMICAL REACTOR AND APPLICATION TO CHLOROPHENOLS IN RIVER WATER [J].

DERUITER, C ;

BOHLE, JF ;

DEJONG, GJ ;

BRINKMAN, UAT ;

FREI, RW .

ANALYTICAL CHEMISTRY, 1988, 60 (07) :666-670

[5] On-line post-column photochemical derivatization in liquid chromatographic-diode-array detection analysis of binary drug mixtures [J].

DiPietra, AM ;

Andrisano, V ;

Gotti, R ;

Cavrini, V .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1996, 14 (8-10) :1191-1199

[6]

Frei R., 1981, Chemical Derivatization in Analytical Chemistry: Chromatography, V1

[7] DETERMINATION OF PRESERVATIVES IN COSMETIC PRODUCTS BY REVERSED-PHASE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY .4. [J].

GAGLIARDI, L ;

CAVAZZUTTI, G ;

TURCHETTO, L ;

MANNA, F ;

TONELLI, D .

JOURNAL OF CHROMATOGRAPHY, 1990, 508 (01) :252-258

[8] Determination of retinoids in galenicals by column liquid chromatography with fluorescence and diode-array detection [J].

Gatti, R ;

Gioia, MG ;

Di Pietra, AM ;

Cini, M .

JOURNAL OF CHROMATOGRAPHY A, 2001, 905 (1-2) :345-350

[9] HPLC-fluorescence determination of chlorocresol and chloroxylenol in pharmaceuticals [J].

Gatti, R ;

Roveri, P ;

Bonazzi, D ;

Cavrini, V .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1997, 16 (03) :405-412

[10]

LINGEMAN H, 1990, DETECTION ORIENTED D, P262

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