Determination of retinoids in galenicals by column liquid chromatography with fluorescence and diode-array detection

被引：13

作者：

Gatti, R ^{[1
]}

Gioia, MG ^{[1
]}

Di Pietra, AM ^{[1
]}

Cini, M ^{[1
]}

机构：

[1] Univ Bologna, Dipartimento Sci Farmaceut, I-40126 Bologna, Italy

来源：

JOURNAL OF CHROMATOGRAPHY A | 2001年 / 905卷 / 1-2期

关键词：

derivatization; LC; retinoids; vitamins;

D O I：

10.1016/S0021-9673(00)00969-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Simple and rapid reversed-phase gradient column liquid chromatography (LC) with fluorescence detection at different wavelengths was developed for the simultaneous analysis of all-trans, 13-cis, 9-cis retinoic acids, vitamin A palmitate and beta -carotene in galenicals. The assay results agreed with those obtained by an LC method with diode-array UV detection. A post-column on-line photochemical reactor (irradiation at 254 and 366 nm) was inserted between the LC column and the fluorescence detector to enhance the performance of the method. Two fluorescence spectra (photoreactor on and off) were obtained for each analyte which proved useful for the unambiguous identification of the various analytes. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：345 / 350

页数：6

共 23 条

[1]

Abahusain MA, 1998, BIOMED CHROMATOGR, V12, P89, DOI 10.1002/(SICI)1099-0801(199803/04)12:2<89::AID-BMC732>3.0.CO

[2]

2-0

[3] Reversed-phase gradient high-performance liquid chromatographic procedure for simultaneous analysis of very polar to nonpolar retinoids, carotenoids and tocopherols in animal and plant samples [J].

Barua, AB ;

Olson, JA .

JOURNAL OF CHROMATOGRAPHY B, 1998, 707 (1-2) :69-79

[4] Properties of retinoids - Structure, handling, and preparation [J].

Barua, AB ;

Furr, HC .

MOLECULAR BIOTECHNOLOGY, 1998, 10 (02) :167-182

[5] SIMULTANEOUS PROFILING AND IDENTIFICATION OF CAROTENOIDS, RETINOLS, AND TOCOPHEROLS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY EQUIPPED WITH 3-DIMENSIONAL PHOTODIODE-ARRAY DETECTION [J].

BENAMOTZ, A .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1995, 18 (14) :2813-2825

[6]

BLAU K, 1993, HDB DERIVATIVES CHRO, pCH15

[7] APPLICABILITY OF A POSTCOLUMN PHOTOCHEMICAL REACTOR IN THE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY OF 34 POLYPHENOLIC COMPOUNDS WITH UV DETECTION [J].

CELA, R ;

LORES, M ;

GARCIA, CM .

JOURNAL OF CHROMATOGRAPHY, 1992, 626 (01) :117-126

[8] Liquid chromatographic analysis of all-rac-alpha-tocopheryl acetate, tocopherols, and retinyl palmitate in SRM 1846 [J].

Chase, GW ;

Eitenmiller, RR ;

Long, AR .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1997, 20 (20) :3317-3327

[9] HPLC-fluorescence determination of unconjugated estrogens in pharmaceuticals [J].

Gatti, R ;

Gioia, MG ;

Di Pietra, AM ;

Cavrini, V .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1998, 18 (1-2) :187-192

[10] HPLC analysis of pharmaceutical estrogens in raw materials and dosage forms [J].

Gatti, R ;

Gotti, R ;

Gioia, MG ;

Cavrini, V .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1998, 17 (02) :337-347

← 1 2 3 →