Novel functional biodegradable polymer:: Synthesis and anticoagulant activity of poly(γ-glutamic acid)sulfonate (γ-PGA-sulfonate)

被引：49

作者：

Matsusaki, M

Serizawa, T

Kishida, A

Endo, T

Akashi, M

机构：

[1] Kagoshima Univ, Fac Engn, Dept Appl Chem & Chem Engn, Kagoshima 8900065, Japan

[2] Natl Cardiovasc Ctr, Res Inst, Dept Biomed Engn, Osaka 5658565, Japan

[3] Yamagata Univ, Fac Engn, Yonezawa, Yamagata 9928510, Japan

来源：

BIOCONJUGATE CHEMISTRY | 2002年 / 13卷 / 01期

关键词：

D O I：

10.1021/bc010008d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

gamma-Poly(glutamic acid) (gamma-PGA), which is produced by Bacillus subtilis, was sulfonated using 2-aminoethane- 1-sufonic acid (taurine) in the presence of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (WSC) to give sulfonated gamma-PGA (gamma-PGA-sulfonate). From H-1 NMR spectroscopy and IR spectroscopy, it was confirmed that taurine was introduced to the side chain of gamma-PGA via an amide linkage. By altering the synthetic conditions, it was possible to control the content of sulfonate in gamma-PGA-sulfonate. Anticoagulant activity was investigated in order to evaluate the biological activity of gamma-PGA-sulfonate by the Lee-White test. The clotting time was prolonged when the concentration of gamma-PGA-sulfonate on the degree of sulfonation was increased. It becomes clear that gamma-PGA-sulfonate is potentially useful for various medical applications, such as drug delivery, tissue engineering, and medical materials.

引用

页码：23 / 28

页数：6

共 30 条

[11]

KUBOTA H, 1992, POLYM PREPR-JAPAN, V41, P1074

[12]

KUNIOKA M, 1994, STUD POLYM SCI, V12, P425

[13]

KUNIOKA M, 1993, POLYM PREPR-JAPAN, V42, P3751

[14] ANTICOAGULANT ACTIVITY OF DEXTRAN DERIVATIVES .1. SYNTHESIS AND CHARACTERIZATION [J].