Quantitative high-performance liquid chromatography analysis of plant phospholipids and glycolipids using light-scattering detection

被引：40

作者：

Picchioni, GA ^{[1
]}

Watada, AE ^{[1
]}

Whitaker, BD ^{[1
]}

机构：

[1] USDA,BELTSVILLE AGR RES CTR,HORT CROPS QUAL LAB,BELTSVILLE,MD 20705

来源：

LIPIDS | 1996年 / 31卷 / 02期

关键词：

D O I：

10.1007/BF02522623

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Application of the evaporative light-scattering principle to quantitative high-performance liquid chromatography (HPLC) analyses of plant membrane lipids has received little study. Light-scattering detection response curves were generated for nine classes of plant membrane phospholipid and glycolipids. Quantitative results obtained by HPLC/light-scattering detection and conventional lipid analytical methods (thin-layer chromatography and lipid-P assay) were in close agreement, confirming the reliability of HPLC/evaporative light-scattering detection (ELSD) analyses. Only three of the nine plant lipid classes gave linear detector response functions above 10 mu g injected lipid mass. This finding contradicts earlier precepts involving light-scattering detection of lipids. At a given mass, appreciable variation in ELSD signal intensity and detection limit was found to exist among the various plant membrane lipid classes. The variation in detector response among plant lipid classes is an important consideration in achieving accurate quantitative results in plant lipid analyses.

引用

页码：217 / 221

页数：5

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