Metabolites of ochratoxins in rat urine and in a culture of Aspergillus ochraceus

被引:45
作者
Xiao, H
Marquardt, RR
Abramson, D
Frohlich, AA
机构
[1] UNIV MANITOBA, DEPT ANIM SCI, WINNIPEG, MB R3T 2N2, CANADA
[2] AGR & AGRI FOOD CANADA, WINNIPEG RES CTR, WINNIPEG, MB R3T 2N2, CANADA
关键词
D O I
10.1128/AEM.62.2.648-655.1996
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We studied the metabolic profile of ochratoxin A (OA) in rats and in a culture of OA-producing Aspergillus ochraceus. Ochratoxin alpha (O alpha), ochratoxin beta (O beta), 4-R-hydroxyochratoxin A (4-R-OH OA), 4-R-hydroxyochratoxin B (4-R-OH OB), and 10-hydroxyochratoxin A (10-OH OA) were isolated from a culture of A. ochraceus and structurally characterized by H-1 nuclear magnetic resonance spectroscopy, mass spectrometry and high-pressure liquid chromatography, 4-R-OH OA and Oa were consistently produced and were the dominant biotransformed metabolites in the fungal culture and in rats treated with OA and ochratoxin C (OC), while the formation of 10-OH OA was conditional in the fungal system. Green fluorescent biomacromolecules were isolated by detergent extraction of the fungal culture followed by cold-acetone precipitation and gel filtration. Acid hydrolysis of the fluorescent macromolecules resulted in the release of several ochratoxins, including O alpha (80%), OA (2%), and OC (5%), and other unidentified fluorescent compounds but not OB and O beta. Cross-reactivity studies of the natural macromolecule conjugates of OA with anti-OA polyclonal antibodies indicated that they were covalently linked to the macromolecules via a group other than the carboxyl group. These studies demonstrated that a fungus can produce some of the same metabolites of OA as the rat and that O alpha, OA, and OC may be covalently linked to fungal macromolecules.
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页码:648 / 655
页数:8
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