Selective repression of YKL-40 by NF-κB in glioma cell lines involves recruitment of histone deacetylase-1 and-2

被引：39

作者：

Bhat, Krishna P. ^{[1
,2
]}

Pelloski, Christopher E. ^{[1
,2
,3
]}

Zhang, Yujian ^{[2
,4
]}

Kim, Se Hoon ^{[1
,2
]}

deLaCruz, Clarissa ^{[1
,2
]}

Rehli, Michael ^{[5
,6
]}

Aldape, Kenneth D. ^{[1
,2
]}

机构：

[1] Univ Texas MD Anderson Canc Ctr, Dept Pathol, Unit 85, Houston, TX 77030 USA

[2] Univ Texas MD Anderson Canc Ctr, Brain Tumor Ctr, Houston, TX 77030 USA

[3] Univ Texas MD Anderson Canc Ctr, Dept Radiat Oncol, Houston, TX 77030 USA

[4] Univ Texas MD Anderson Canc Ctr, Dept Neurosurg, Houston, TX 77030 USA

[5] Univ Regensburg, Dept Hematol & Oncol, D-93042 Regensburg, Germany

[6] Yonsei Univ, Coll Med, Dept Pathol, Seoul, South Korea

来源：

FEBS LETTERS | 2008年 / 582卷 / 21-22期

关键词：

YKL-40; NF-kappa B; glioma; HDAC;

D O I：

10.1016/j.febslet.2008.08.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Here we show that in contrast to other cancer types, tumor necrosis factor (TNF)-alpha suppresses YKL-40 expression in glioma cell lines in a nuclear factor kappa B (NF-kappa B) dependent manner. Even though TNF-alpha causes recruitment of p65 and p50 subunits of NF-kappa B to the YKL-40 promoter in all cell types, recruitment of histone deacetylases (HDAC)-1 and -2, and a consequent deacetylation of histone H3 at the YKL-40 promoter occurs only in glioma cells. Importantly, using chromatin immunoprecipitation assays in frozen glioblastoma multiforme tissues, we show that YKL-40 levels decrease consistent with HDAC1 recruitment despite high levels of nuclear p-p65. This study presents a paradigm for NF-kappa B regulation of one of its targets in a strict cell type specific manner. Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：3193 / 3200

页数：8

共 30 条

[1]

The p65 (RelA) subunit of NF-κB interacts with the histone deacetylase (HDAC) corepressors HDAC1 and HDAC2 to negatively regulate gene expression [J].