A hydrogel scaffold that maintains viability and supports differentiation of dental pulp stem cells

被引:147
作者
Cavalcanti, Bruno N. [1 ,2 ]
Zeitlin, Benjamin D. [1 ,3 ]
Noer, Jacques E. [1 ,4 ,5 ]
机构
[1] Univ Michigan, Sch Dent, Dept Cariol, Ann Arbor, MI 48109 USA
[2] Sao Paulo State Univ, Dept Restorat Dent, Sao Jose Dos Campos, Brazil
[3] Univ Pacific, Arthur A Dugoni Sch Dent, San Francisco, CA USA
[4] Univ Michigan, Coll Engn, Dept Biomed Engn, Ann Arbor, MI 48109 USA
[5] Univ Michigan, Sch Med, Dept Otolaryngol, Ann Arbor, MI 48109 USA
关键词
Tissue engineering; Hydrogel; Dental pulp; Regenerative Endodontics; Odontoblast; Stem cells; IN-VITRO; ENDOTHELIAL-CELLS; BONE REGENERATION; ANGIOGENESIS; MATRIX; DSPP; VIVO;
D O I
10.1016/j.dental.2012.08.002
中图分类号
R78 [口腔科学];
学科分类号
100302 [口腔临床医学];
摘要
Objectives. The clinical translation of stem cell-based Regenerative Endodontics demands further development of suitable injectable scaffolds. Puramatrix (TM) is a defined, self-assembling peptide hydrogel which instantaneously polymerizes under normal physiological conditions. Here, we assessed the compatibility of Puramatrix (TM) with dental pulp stem cell (DPSC) growth and differentiation. Methods. DPSC cells were grown in 0.05-0.25% Puramatrix (TM). Cell viability was measured colorimetrically using the WST-1 assay. Cell morphology was observed in 3D modeling using confocal microscopy. In addition, we used the human tooth slice model with Puramatrix (TM) to verify DPSC differentiation into odontoblast-like cells, as measured by expression of DSPP and DMP-1. Results. DPSC survived and proliferated in Puramatrix (TM) for at least three weeks in culture. Confocal microscopy revealed that cells seeded in Puramatrix (TM) presented morphological features of healthy cells, and some cells exhibited cytoplasmic elongations. Notably, after 21 days in tooth slices containing Puramatrix (TM), DPSC cells expressed DMP-1 and DSPP, putative markers of odontoblastic differentiation. Significance. Collectively, these data suggest that self-assembling peptide hydrogels might be useful injectable scaffolds for stem cell-based Regenerative Endodontics. (c) 2012 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:97 / 102
页数:6
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