DNA probes using fluorescence resonance energy transfer (FRET): Designs and applications

被引：280

作者：

Didenko, VV ^{[1
]}

机构：

[1] Baylor Coll Med, Houston, TX 77030 USA

来源：

BIOTECHNIQUES | 2001年 / 31卷 / 05期

关键词：

D O I：

10.2144/01315rv02

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence resonance energy transfer (FRET) is widely used in biomedical research as a reporter method. Oligonucleotides with a DNA backbone and one or several chromophore tags have found multiple applications as FRET probes. They are especially advantageous for the real-time monitoring of biochemical reactions and in vivo studies. This paper reviews the design and applications of various DNA-based probes that use FRET. The approaches used in the design of new DNA FRET probes are discussed.

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页码：1106 / +

页数：14

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