Efficient incorporation of unnatural amino acids into proteins in Escherichia coli

被引：251

作者：

Ryu, YH

Schultz, PG

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

NATURE METHODS | 2006年 / 3卷 / 04期

关键词：

D O I：

10.1038/NMETH864

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a single-plasmid system for the efficient bacterial, expression of mutant proteins containing unnatural amino acids at specific sites designated by amber nonsense codons. In this system, multiple copies of a gene encoding an amber suppressor tRNA derived from a Methanocaldococcus jannaschii tyrosyl-tRNA (MjtRNA(CUA)(Tyr)) are expressed under control of the proK promoter and terminator, and a gene encoding the desired mutant M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS) is expressed under control of a mutant glnS (glnS') promoter.

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页码：263 / 265

页数：3

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