Reconstruction and analysis of a genome-scale metabolic model of the vitamin C producing industrial strain Ketogulonicigenium vulgare WSH-001

被引：32

作者：

Zou, Wei ^{[1
,3
]}

Liu, Liming ^{[1
,2
,3
]}

Zhang, Jing ^{[4
]}

Yang, Haoru ^{[1
,3
]}

Zhou, Maoda ^{[1
,3
]}

Hua, Qiang ^{[2
]}

Chen, Jian ^{[1
,3
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China

[2] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[3] Jiangnan Univ, Minist Educ, Key Lab Ind Biotechnol, Wuxi 214122, Jiangsu, Peoples R China

[4] Sichuan Univ Sci & Engn, Coll Bioengn, Zigong 643000, Sichuan, Peoples R China

来源：

JOURNAL OF BIOTECHNOLOGY | 2012年 / 161卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Genome-scale metabolic model; Ketogulonicigenium vulgare; 2-keto-L-Gulonic acid; Sulfate metabolism; Vitamin C; L-ASCORBIC-ACID; 2-KETO-L-GULONIC ACID; L-SORBOSE; BACILLUS-MEGATERIUM; DEHYDROGENASE; ANNOTATION; SEQUENCE; DATABASE; SERVER;

D O I：

10.1016/j.jbiotec.2012.05.015

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Ketogulonicigenium vulgare WSH001 is an industrial strain commonly used in the vitamin C producing industry. In order to acquire a comprehensive understanding of its physiological characteristics, a genome-scale metabolic model of K. vulgare WSH001, iWZ663, including 830 reactions, 649 metabolites, and 663 genes, was reconstructed by genome annotation and literature mining. This model was capable of predicting quantitatively the growth of K. vulgare under l-sorbose fermentation conditions and the results agreed well with experimental data. Furthermore, phenotypic features, such as the defect in sulfate metabolism hampering the syntheses of l-cysteine, l-methionine, coenzyme A (CoA), and glutathione, were investigated and provided an explanation for the poor growth of K. vulgare in monoculture. The model presented here provides a validated platform that can be used to understand and manipulate the phenotype of K. vulgare to further improve 2-KLG production efficiency. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：42 / 48

页数：7

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