Characterization of Toxoplasma gondii isolates in free-range chickens from Amazon, Brazil

被引：66

作者：

Dubey, JP ^{[1
]}

Gennari, SM ^{[1
]}

Labruna, MB ^{[1
]}

Camargo, LMA ^{[1
]}

Vianna, MCB ^{[1
]}

Marcet, PL ^{[1
]}

Lehmann, T ^{[1
]}

机构：

[1] USDA ARS, Anim & Nat Resources Inst, Anim Parasit Dis Lab, Beltsville, MD 20705 USA

来源：

JOURNAL OF PARASITOLOGY | 2006年 / 92卷 / 01期

关键词：

D O I：

10.1645/GE-655R.1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The prevalence of Toxoplasma gondii in free-ranging chickens is a good indicator of the prevalence of 7. gondii oocysts in the soil because chickens feed from the ground. The prevalence of 7. gondii in 50 free-range chickens (Gallus domesticus) from Amazon, Brazil, was determined. Antibodies to T. gondii were assayed by the modified agglutination test (MAT) and found in 33 (66%) chickens with titers of: 1:5 in 3, 1:10 in 2, 1:20 in 1, 1:40 in 1, 1:80 in 2, 1:160 in 5.1:200 in 9, 1:400 in 5, 1:800 in 2, 1:1.600 in 2. and 1:3,200 or higher in 1. Hearts and brains of 33 seropositive chickens were bioassayed individually in mice. Tissues from 17 seronegative chickens were pooled and fed to 2 7. gondii-free cats. Feces of cats were examined for oocysts, but none was found. Toxoplasma gondii was isolated from 24 chickens with MAT titers of 1:5 or higher. Genotyping of these 24 7. gondii isolates by polymorphisms at the SAG2 locus indicated that 14 were type 1, and 10 were type 111; the absence of type 11 strains from Brazil was confirmed. Fifty percent of the infected mice died of toxoplasmosis, irrespective of the genotype.

引用

页码：36 / 40

页数：5

共 50 条

[1] Genetic diversity, clonality and sexuality in Toxoplasma gondii [J].

Ajzenberg, D ;

Bañuls, AL ;

Su, C ;

Dumètre, A ;

Demar, M ;

Carme, B ;

Dardé, ML .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2004, 34 (10) :1185-1196

[2] Genotype of 86 Toxoplasma gondii isolates associated with human congenital toxoplasmosis, and correlation with clinical findings [J].

Ajzenberg, D ;

Cogné, N ;

Paris, L ;

Bessières, MH ;

Thulliez, P ;

Filisetti, D ;

Pelloux, H ;

Marty, P ;

Dardé, ML .

JOURNAL OF INFECTIOUS DISEASES, 2002, 186 (05) :684-689

[3] Molecular evidence for multiple Toxoplasma gondii infections in individual patients in England and Wales:: public health implications [J].

Aspinall, TV ;

Guy, EC ;

Roberts, KE ;

Joynson, DHM ;

Hyde, JE ;

Sims, PFG .

INTERNATIONAL JOURNAL FOR PARASITOLOGY, 2003, 33 (01) :97-103

[4]

Bahia-Oliveira LMG, 2003, EMERG INFECT DIS, V9, P55

[5]

Baruzzi R. G., 1970, Revta Inst. Med. trop. S. Paulo, V12, P93

[6] Population biology of Toxoplasma gondii and its relevance to human infection:: do different strains cause different disease? [J].

Boothroyd, JC ;

Grigg, ME .

CURRENT OPINION IN MICROBIOLOGY, 2002, 5 (04) :438-442

[7] A rural community in a Brazilian Western Amazonian region: Some demographic and epidemiological patterns [J].

Camargo, LMA ;

Moura, MM ;

Engracia, V ;

Pagotto, RC ;

Basano, SA ;

da Silva, LHP ;

Camargo, EP ;

Beiguelman, B ;

Krieger, H .

MEMORIAS DO INSTITUTO OSWALDO CRUZ, 2002, 97 (02) :193-195

[8] Genotyping of Toxoplasma gondii strains isolated from dogs with neurological signs [J].

da Silva, AV ;

Pezerico, SB ;

de Lim, VY ;

Moretti, LD ;

Pinheiro, JP ;

Tanaka, EM ;

Ribeiro, MG ;

Langoni, H .

VETERINARY PARASITOLOGY, 2005, 127 (01) :23-27

[9]

DEMOURA L, 2003, IN PRESS EMERGING IN

[10]

Dubey J. P., 1988, TOXOPLASMOSIS ANIMAL

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