Expression of recombinant human ceruloplasmin - An absolute requirement for splicing signals in the expression cassette

被引：22

作者：

Rafiq, M ^{[1
]}

Suen, CKM ^{[1
]}

Choudhury, N ^{[1
]}

Joannou, CL ^{[1
]}

White, KN ^{[1
]}

Evans, RW ^{[1
]}

机构：

[1] UNITED MED & DENT SCH,GUYS HOSP,DIV BIOCHEM & MOL BIOL,LONDON SE1 9RT,ENGLAND

来源：

FEBS LETTERS | 1997年 / 407卷 / 02期

基金：

英国惠康基金;

关键词：

human ceruloplasmin; intron; expression cassette; mRNA export; in situ hybridization;

D O I：

10.1016/S0014-5793(97)00325-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the successful expression of recombinant human ceruloplasmin which was made possible by inclusion of splicing signals in the expression vector. Ceruloplasmin cDNA expressed from the vector pNUT in baby hamster kidney cells gave protein yields of 0.03 mg/l which increased to 15 mg/l with splicing signals present, The defect in expression from the intronless cDNA is due to complete retention of ceruloplasmin mRNA in cell nuclei. The block to cytoplasmic export is alleviated by splicing signals, allowing full expression of the mRNA. (C) 1997 Federation of European Biochemical Societies.

引用

页码：132 / 136

页数：5

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