Assessment of fluorescein diacetate hydrolysis as a measure of total esterase activity in natural stream sediment biofilms

被引:98
作者
Battin, TJ
机构
[1] Department of Ecology, University of Vienna, A-1090 Vienna
关键词
fluorescein diacetate; biofilm esterase activity; stream sediment; bacteria; thymidine; glucosidase; electron transport system; EPS;
D O I
10.1016/S0048-9697(97)05441-7
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
I applied the fluorescein diacetate (FDA) hydrolysis technique as a rapid and sensitive estimator of total esterase activity in stream sediment biofilms. I investigated the effects of temperature, pH and incubation time and optimized the assay for low blanks and high fluorescein extraction. The FDA procedure was precise (c.v. = 4.15%) and could detect 10 nM fluorescein. Spatial patterns of esterase activity within stream sediment biofilms correlated with electron transport system activity, bacterial thymidine incorporation, glucosidase activity and chlorophyll a. As such, I suggest the modified and optimized technique as applicable to the investigation of total stream bioflim esterase activity. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:51 / 60
页数:10
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